For Research Use Only. Not for use in diagnostic procedures. Others LightMix Kit Factor II RUO LightMix® Kit Factor II 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000649 Detection Kit for Factor II G20210A mutation 10 097 647 001 10097647001 LightMix Factor II LightMix Factor II 04260159334999 Reagents, kits 40-0593-RUO 64 reactions Not Available false Analytical SensitivityDetection of serial dilutions of several heterozygous human genomic DNAs has revealed that the limit of detection of the present kit is 250 copies (1.5 ng).Analytical SpecificityThe specificity to the target gene and the suitability of the PCR amplification employed in the present test was demonstrated by sequencing of the amplicon. en Using PCR methodology, a 106 bp fragment of the Factor II gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® oligomer binding to the region spanning the mutation site.During the melting curve analysis, the temperature is slowly increased. The probe leaves at a specific melting temperature (Tm) causing a fluorescence decrease. Any mismatch covered by the probe destabilizes the hybrid and lowers the Tm.In this product, the probe matches the sequence of the wild type genotype and the presence of a mutation variant will yield a reduced Tm.Reading of the genotype results is based on the melting temperatures compared to the supplied standards. If permitted by the instrument software reading of the genotype results can be achieved by the automated genotyping module (instrument-dependent: software module 'Melt Curve Genotyping').Automated genotyping results must be reviewed by eye for deviating curves and intermediate melting point temperatures. In case that automated typing fails to report consistent genotype results, the genotype must be deduced from the melting temperatures following the criteria described in chapter 8. en This kit detects the G20210A mutation of the coagulation Factor II gene from human genomic DNA extracts. A 106 bp fragment of the Factor II gene is amplified with specific primers. The PCR fragment is analyzed using an internally labeled SimpleProbe® (465-510 channel) binding to the region spanning the mutation site. For use on Roche LightCycler® systems. en

LightMix^® Kit Factor II

Detection Kit for Factor II G20210A mutation

RUO

User Profile

Select your user profile

LightMix^® Kit Factor II

Overview

Detailed Specifications

Ordering Information

Compatible Instruments

Technical Documents

Access important product documentation including relevant certificates and other resources.