For life science research only. Not for use in diagnostic procedures. Others LightMix Kit Polyomaviruses JC and BK LSR LightMix® Kit Polyomaviruses JC and BK 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. TIBMOLBIOL-0013 Kit with reagents for the quantitative detection of Polyomavirus JC and BK DNA using the Roche Diagnostics LightCycler® 1.x / 2.0 / 480 II (not LC480 1. version) Instruments or cobas z 480 Analyzer. 05 879 540 001 5 879 540 001 05879540001 5879540001 05879540001 LightMix Polyomavirus JC BK IC LightMix Polyomavirus JC BK IC 04260159330571 Reagents, kits 40-0203-32 96 reactions Not Available false This kit detects Polyomaviruses JC (Human polyomavirus 2) and BK (Human polyomavirus 1) from DNA extracts, and includes a internal positive control (IC). A 172 bp fragment (JCV) and/or 175 bp fragment (BKV) of the Small t-Antigen gene are amplified with specific primers and detected with probes labeled with LC690 (705 channel) for JCV or with LC640 (640 channel) for BKV. An additional PCR product of 658 bp is formed from the internal control DNA (IC). The detection probe or the IC is labeled with a LC640 dye. For use on Roche LightCycler® systems. en PCR results are obtained within 50 minutes (50 cycles and melting curve) with the LightCycler® 1.x / 2.0 Instruments and within 80 minutes (50 cycles and melting curve) with '480' plate based Instruments.SensitivityThese reagents detect 10 copies of Polyomaviruses DNA using the Roche ‘LightCycler® FastStart DNA Master HybProbe’ (in an exemplary system, using cloned targets as reference). The detection limit for clinical specimen was determined to be 7,000 copies/ml from plasma and 15,000 copies/ml from urine. The test had a specificity of 100%, sensitivity of 97.9% and showed a high precision (coefficient of variation < 4%)2.Measuring rangeThe linear measuring range of the assay is 102 to 106 copies of Polyomaviruses JC/BK DNA per reactions, using the Roche ‘LightCycler® FastStart DNA Master HybProbe’ with the LightCycler® 1.x / 2.0 / plate based Instruments and a cloned standard. en

LightMix^® Kit Polyomaviruses JC and BK

Kit with reagents for the quantitative detection of Polyomavirus JC and BK DNA using the Roche Diagnostics LightCycler^® 1.x / 2.0 / 480 II (not LC480 1. version) Instruments or cobas z 480 Analyzer.

LSR

User Profile

Select your user profile

LightMix^® Kit Polyomaviruses JC and BK

Overview

Detailed Specifications

Ordering Information

Compatible Instruments

Technical Documents

Access important product documentation including relevant certificates and other resources.