For in vitro diagnostic use. Others LightMix Kit TREC SMA HBB Newborn IVD LightMix® Kit TREC SMA HBB Newborn PID00000321 Detection Kit with primer/probes for 384 (or 192) PCR reactions [dried] for use with the Roche Diagnostics LightCycler® instruments and LightCycler® Multiplex DNA Master 09 802 533 001 9 802 533 001 09802533001 9802533001 09802533001 LightMix TREC SMA HBB Newborn LightMix TREC SMA HBB Newborn 04260159332377 Reagents, kits 40-0621-44 384 reactions (10µL)192 reactions (20µL) Not Available false The LightMix® TREC SMA HBB Newborn kit is an in-vitro diagnostic test intended to be used to screen genomic nucleic acid (DNA) extracted from dried blood spots (DBS) from newborns for (1) the absence of T-cell receptor excision circles (TREC), a sign for severe combined immunodeficiency disease (SCID), (2) homozygous deletions of survival motor neuron 1 (SMN1) exon 7, the most predominant cause for severe type 1 spinal muscular atrophy (SMA), and optional (3) for mutations in the region of codon 6 of the beta globin gene, commonly associated with sickle cell disease (SCD) and beta-thalassemia. In the case that the national regulations do not permit genetic thalassemia testing, the melting analysis can be skipped. en The LightMix® TREC SMA HBB Newborn kit is an in-vitro diagnostic test for the simultaneous detection of the absence of TREC, deletions in SMN1 gene and mutations in codon 6 region of the beta globin gene. Genomic DNA extracted from DBS on Guthrie cards, TNF cards or Whatman Genecards is analyzed by a multiplex PCR, a fragment of the genes of interest is amplified with specific primers and detected using labelled probes. The PCR combines the amplification of a 91 bp long fragment for detection of TREC in the 610 channel and an 82 bp fragment of SMN1 gene detected in the 580 channel. A 90 bp long fragment from the beta globin gene is also amplified and detected in the 530 channel using a SimpleProbe® with melting curve analysis. Both TREC and SMN1 analysis detect target sequences present only in wild-type samples, with signal lost in samples with deleted genes. SMN1 and TREC serve as a mutual control, as it is unlikely that both SMN1 and TREC will be absent in the same sample. Users intending to test for KREC or include Actin as the control assay can combine the respective assays within one multiplex PCR. en

LightMix^® Kit TREC SMA HBB Newborn

Detection Kit with primer/probes for 384 (or 192) PCR reactions [dried] for use with the Roche Diagnostics LightCycler^®instruments and LightCycler^® Multiplex DNA Master

IVD

User profile

Select your user profile

LightMix^® Kit TREC SMA HBB Newborn

Overview

Ordering information

Technical documents

Access important product documentation including relevant certificates and other resources.

Compatible products

Related products

Contact us