For Research Use Only. Not for use in diagnostic procedures. Others LightMix Kit TREC SMN1 HBB RUO LightMix® Kit TREC SMA HBB PID00000395 Detection Kit with primer/probes for 384 (or 192) PCR reactions [dried] for use with the Roche Diagnostics LightCycler® 480 instruments and LightCycler® Multiplex DNA Master 09 882 189 001 9 882 189 001 09882189001 9882189001 09882189001 LightMix Kit TREC SMA HBB Newborn LightMix Kit TREC SMA HBB Newborn Reagents, kits 40-0621-RUO 192/384 reactions Not Available false Genomic DNA extracted from dried blood spots (DBS) is analyzed by a multiplex PCR, that combines the amplification of a 91 bp long fragment for detection of TREC, detected in the 610 channel, an 82 bp fragment for detection of SMN1, detected in the 580 channel and a 90 bp long fragment from the beta Globin gene, detected in the 530 channel running a melting analysis on the PCR product.TREC and SMN1 analysis are based on visualizing no amplification as the assay target, is absent or deleted. SMN1 is used in place of the control assay Actin, as it is unlikely that both SMN1 and TREC will be absent.Users intending to test for KREC or include Actin can add the respective assays into the one multiplex PCR. en Analytical SensitivityThe sensitivity has been determined in multiplex PCR to be in the range of 1-2 copies for TREC and 8 copies for SMN1. Every lot is verified to have a detection limit of at least 10 copies.The test requires a minimum of extracted DNA as measured by the Cp value of the SMN1 PCR.Analytical SpecificityThe specificity to the target gene and the suitability of the PCR amplification employed in the present test was demonstrated by sequencing of the amplicon. en This kit detects the absence of T-cell receptor excision circles (TREC), homozygous deletions of SMN1, and optional mutations in codon 6 of the beta globin gene (HBB) from human genomic DNA extracts. A 91 bp long fragment for detection of TREC (610 channel), an 82 bp fragment for detection of SMN1 (580 channel), and a 90 bp long fragment from the beta Globin gene (530 channel) are differentiated by running a melting analysis on the PCR product. For use on Roche LightCycler® systems. en

LightMix^® Kit TREC SMA HBB

Detection Kit with primer/probes for 384 (or 192) PCR reactions [dried] for use with the Roche Diagnostics LightCycler^® 480 instruments and LightCycler^® Multiplex DNA Master

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