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This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This antibody is intended for in vitro diagnostic (IVD) use.
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"Value": "Melanoma Triple Cocktail (HMB45+A103+T311) Primary Antibody (Melanoma Triple Cocktail (HMB45+A103+T311) antibody) is an antibody cocktail of anti-Melanosome (HMB45), anti-MART-1/melan A (A103), and anti-Tyrosinase (T311) mouse monoclonal antibodies. Premelanosome protein (PMEL), melan A, and tyrosinase are melanocyte markers and are expressed in the majority of melanocytic lesions.1,2,3 A cocktail of antibodies directed against premelanosome protein (PMEL), melan A, and tyrosinase has been reported to provide advantages in sensitivity, over the use of the individual antibodies alone, in the detection of melanocytic lesions, while still maintaining good specificity.4 In addition, in cases of lymph node and cutaneous metastases this cocktail was as sensitive as anti-S100.4
Melanoma Triple Cocktail (HMB45+A103+T311) antibody may be used as a melanocyte marker to aid in the differential diagnosis of melanocytic versus non-melanocytic tumors. The staining pattern is cytoplasmic.
1. Chen YT, Stockert E, Jungbluth A, et al. Serological analysis of Melan-A (MART-1), a melanocyte-specific protein homogeneously expressed in human melanomas. Proc Natl Acad Sci USA. 1996;93:5915-5919.
2. Duray PH, Palazzo J, Gown AM, et al. Melanoma cell heterogeneity. A study of two monoclonal antibodies compared with S-100 in paraffin sections. Cancer. 1988;61:2460-2468.
3. Hofbauer GF, Kamarashev J, Geertsen R et al. Tyrosinase immunoreactivity in formalin-fixed, paraffin-embedded primary and metastatic melanoma: frequency and distribution. J Cutan Pathol. 1998;25:204-209.
4. Orchard G. Evaluation of melanocytic neoplasms: application of a pan-melanoma antibody cocktail. Br J Biomed Sci. 2002;59:196-202.",
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One 5 mL dispenser of Melanoma Triple Cocktail (HMB45+A103+T311) antibody contains approximately 23.5 μg of a mouse monoclonal antibody cocktail.
The antibody cocktail is diluted in Tris-HCl dilution buffer with a carrier protein and 0.10% ProClin 300, a preservative.
Specific antibody concentration is approximately 4.7 μg/mL. There is no known non-specific antibody reactivity observed in this product.
Melanoma Triple Cocktail (HMB45+A103+T311) antibody is a cocktail of monoclonal antibodies produced as cell culture supernatant.
Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.",
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This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This antibody is intended for in vitro diagnostic (IVD) use.
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