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Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "CONFIRM anti-Melanosome (HMB45) antibody contains sufficient reagent for 50 tests.

One 5 mL dispenser of CONFIRM anti-Melanosome (HMB45) antibody contains approximately 2.5 μg of a mouse monoclonal antibody.
The antibody is diluted in Tris-HCI with carrier protein and 0.10% ProClin 300, a preservative. Specific antibody concentration is approximately 0.5 μg/mL. There is no known nonspecific antibody reactivity observed in this product.
There is a trace (~2%) of fetal calf serum of U.S. origin from the stock solution. Refer to the appropriateVENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, MaterialandMethods, SpecimenCollection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "CONFIRM anti-Melanosome (HMB45) is a mouse monoclonal antibody intended for laboratory use in the qualitative immunohistochemical detection of premelanosome protein (PMEL) by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological exanimation, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostics.", "Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Human melanoma black (HMB45) is a monoclonal antibody raised against a 100 kDa type 1 transmembrane glycoprotein, premelanosome protein (PMEL). PMEL was discovered in numerous contexts, and as a result, has multiple aliases, the most common being: gp100, PMEL17, SILV, and SI.^1,2 PMEL is responsible for the formation of fibrillar sheets within early stage melanosomes.^3,4 The fibrillar sheets serve as a template upon which melanin polymers are deposited in later stage melanosomes.^2,4 Alternative splicing leads to the generation of four PMEL isoforms, all of which react with monoclonal antibodies commonly used for IHC assays.^2,3,4,5,6,7 Varying degrees of hypopigmentation and pigment cell inviability are seen in animals that either lack PMEL expression or express mutant PMEL variants, indicating that PMEL fibrils are required for optimal pigment cell function.^3,4,8,9 PMEL expression is restricted to cells of the melanocytic lineage. Cutaneous fetal melanocytes and prenatal and infantile retinal pigmented epithelium express PMEL.^10,11 Whereas, cells of intradermal nevi or normal adult melanocytes do not, regardless of the degree of pigmentation.^12,13 PMEL is expressed in the cytoplasm of neoplastic melanocytic cells, including the cells of junctional nevi and malignant melanoma.¹³ It is suggested that antibodies specific for PMEL recognize epitopes expressed only in proliferating melanocytes, both benign and malignant, which explains why fetal and malignant melanocytes, which are proliferative cells, react with PMEL antibodies while adult melanocytes are unreactive.¹³ Additional studies indicate that PMEL immunolabeling is reduced in mature melanosomes, suggesting that deposition of melanin in later stage melanosomes masks the PMEL epitopes on the fibrillar sheets.^2,4
Detection of PMEL by immunohistochemistry (IHC) with the CONFIRM anti-Melanosome (HMB45) Mouse Monoclonal Primary Antibody (CONFIRM anti-Melanosome (HMB45) antibody) may be used as a melanocyte marker to aid in the differential diagnosis of melanocytic versus non-melanocytic tumors. It may be used as part of a panel of IHC studies. The staining pattern is cytoplasmic.

1. Louros NN, Baltoumas FA, Hamodrakas SJ, Iconomidou VA. A beta-solenoid model of the Pmel17 repeat domain: insights to the formation of functional amyloid fibrils. J Comput Aided Mol Des. 2016;30(2):153-164.
2. Theos AC, Truschel ST, Raposo G, Marks MS. The Silver locus product Pmel17/gp100/Silv/ME20: controversial in name and in function. Pigment Cell Res. 2005;18(5):322-336.
3. Bissig C, Rochin L, van Niel G. PMEL Amyloid Fibril Formation: The Bright Steps of Pigmentation. Int J Mol Sci. 2016;17(9).
4. Watt B, van Niel G, Raposo G, Marks MS. PMEL: a pigment cell-specific model for functional amyloid formation. Pigment Cell Melanoma Res. 2013;26(3):300-315.
5. College of American Pathologists (CAP) Protocol for the Examination of Specimens From Patients With Melanoma of the Skin. 2017.
6. Adema GJ, de Boer AJ, Vogel AM, Loenen WA, Figdor CG. Molecular characterization of the melanocyte lineage-specific antigen gp100. J Biol Chem. 1994;269(31):20126-20133.
7. Nichols SE, Harper DC, Berson JF, Marks MS. A novel splice variant of Pmel17 expressed by human melanocytes and melanoma cells lacking some of the internal repeats. J Invest Dermatol. 2003;121(4):821-830.
8. Hellstrom AR, Watt B, Fard SS, et al. Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation. PLoS Genet. 2011;7(9):e1002285.
9. Theos AC, Berson JF, Theos SC, et al. Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17. Mol Biol Cell. 2006;17(8):3598-3612.
10. Kapur RP, Bigler SA, Skelly M, Gown AM. Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes. J Histochem Cytochem. 1992;40(2):207-212.
11. Ordonez NG. Value of melanocytic-associated immunohistochemical markers in the diagnosis of malignant melanoma: a review and update. Human Pathology. 2014;45(2):191-205.
12. Esclamado RM, Gown AM, Vogel AM. Unique proteins defined by monoclonal antibodies specific for human melanoma. Some potential clinical applications. Am J Surg. 1986;152(4):376-385.
13. Gown AM, Vogel AM, Hoak D, Gough F, McNutt MA. Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol. 1986;123(2):195-203.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "CONFIRM anti-Melanosome (HMB45) antibody binds to melanosomes in active melanocytes. This antibody can be visualized using ultraView Universal Alkaline Phosphatase Red Detection Kit (Cat. No. 760-501 / 05269814001). Refer to the respective method sheet for further information.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "CONFIRM anti-Melanosome (HMB45) is a mouse monoclonal antibody intended for laboratory use in the qualitative immunohistochemical detection of premelanosome protein (PMEL) by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument.

This product should be interpreted by a qualified pathologist in conjunction with histological exanimation, relevant clinical information, and proper controls.

This antibody is intended for in vitro diagnostics.", "Language": "en", "Country": "XG", "Code": "Product Purpose" } ] } } ] }