Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Nucleus Others Myogenin F5D IVD Rhabdomyosarcoma Myogenin (F5D) Mouse Monoclonal Antibody RTD001057 Mouse F5D 760-2832 Myogenin (F5D) 00841683100416 50 tests true NexES, Reagent, Cell MArque, Myogenin 05268290001 Not Available Reagents, kits en Myogenin (F5D) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the myogenin protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This antibody is intended for in vitro diagnostic (IVD) use. en Myogenin (F5D) Mouse Monoclonal Antibody (this antibody) may be used as the primaryantibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissuesections. In general, immunohistochemical staining allows the visualization of antigensvia the sequential application of a specific antibody (primary antibody) to the antigen, asecondary antibody (link antibody) to the primary antibody, an enzyme complex and achromogenic substrate with interposed washing steps. The enzymatic activation of thechromogen results in a visible reaction product at the antigen site. The specimen may then becounterstained and a coverslip applied. Results are interpreted using a light microscope andaid in the differential diagnosis of pathophysiological processes, which may or may not beassociated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.