For in vitro diagnostic use. Others OptiView DAB IHC Detection Kit IVD OptiView DAB IHC Detection Kit RTD001078 06396500001 OptiView DAB Detection Kit OptiView DAB IHC Detection Kit 04015630984039 Reagents, kits 760-700 250 tests Not Available true OptiView DAB IHC Detection Kit (OptiView) is an indirect, biotin-free system for detecting mouse IgG, mouse IgM, and rabbit primary antibodies. The kit is intended to identify targets by immunohistochemistry (IHC) in sections of formalin-fixed, paraffin-embedded and frozen tissue that are stained on the VENTANA automated slide stainers and visualized by light microscopy. The clinical interpretation of any staining, or the absence of staining, must be complemented by morphological studies and evaluation of proper controls.This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.This product is intended for in vitro diagnostic (IVD) use. en The OptiView DAB IHC Detection Kit detects specific mouse and rabbit primary antibodies bound to an antigen in formalin-fixed, paraffin-embedded (FFPE) or frozen tissue sections. The specific antibody is located by a specific secondary antibody that is bound by an enzyme-labeled tertiary antibody. The complex is then visualized with hydrogen peroxide substrate and 3, 3’-diaminobenzidine tetrahydrochloride (DAB) chromogen, which produces a brown precipitate that is readily observed by light microscopy.The staining protocol consists of numerous steps in which reagents are incubated for predetermined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents from the slide.1 Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.For more detailed information on instrument operation, refer to the appropriate User Guide. en