Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Nucleus Others Phosphohistone H3 PHH3 IVD Tonsil Phosphohistone H3 (PHH3) Rabbit Polyclonal Antibody RTD001090 Rabbit 06 433 405 001 6 433 405 001 06433405001 6433405001 06433405001 PHH3 (polyclonal) PAb, Cell Marque Phosphohistone H3 (PHH3) 00841683101208 Reagents, kits 50 tests Not Available true Phosphohistone H3 (PHH3) Rabbit Polyclonal Primary Antibody is intended for laboratory use in the detection of the Phosphohistone H3 (PHH3) protein in formalin-fixed, paraffinembedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en Phosphohistone H3 Rabbit Polyclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual. en

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