For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Bordetella RUO UC-TIB-Bordetella 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001077 10 604 921 001 10604921001 UC-TIB-Bordetella UC-TIB-Bordetella Reagents, kits 192 reactions Not Available false The limit of detection (LoD) for UC-TIB-Bordetella was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 μL sample volume). The study demonstrates that the UC-TIB-Bordetella detects at least 90 gene copies or less per mL for hIS1001, 50 copies or less per mL for IS481, 45 copies or less per mL for pIS1001 and 70 genome equivalent copies or less per mL for bfrA, determined by LOGIT analysis with a hit rate of 95%. en Bacterial DNA is analyzed by real-time PCR, that amplifies a 142 bp long fragment of hIS1001 gene from B. holmesii analyzed with a Coumarin-labeled probe in channel 01, a 69 bp long fragment of IS481 gene from B. pertussis/holmesii analyzed with a FAM-labeled probe in channel 02, a 80 bp long fragment of pIS1001 gene from B. parapertussis (also present in some B. bronchiseptica) analyzed with a HEX-labeled probe in channel 03 and a 72 bp long fragment of bfrA gene from B. bronchiseptica analyzed with a LC640 labeled probe in channel 04. The targets IS481, hIS1001 and pIS1001 are multi-copy genes supporting a higher sensitivity compared to the bfrA gene. The (+) Ctrl comprises plasmids with a 396 bp long synthetic fragment of hIS1001 gene from B.holmesii, a 363 bp long synthetic fragment of IS481 gene from B.pertussis, a 495 bp long synthetic fragment of pIS1001 gene from B.parapertussis and a 224 bp long synthetic fragment of bfrA gene from B.bronchiseptica (Accession number: AY786982 position 2 - 398 for hIS1001 gene, M28220 position 695 - 1057 for IS481 gene, X66858 position 1 - 495 for IS1001 gene and U56084 position 620 - 843 for bfrA gene respectively). en UC-TIB-Bordetella is an automated qualitative in vitro test for the detection of Bordetella spp. DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Bordetella holmesii, Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. en

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