For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-GAS-AH RUO UC-TIB-GAS-AH 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001078 10 604 905 001 10604905001 UC-TIB-GAS-AH UC-TIB-GAS-AH Reagents, kits 192 reactions Not Available false Bacterial DNA is analyzed by a real-time PCR that amplifies a 95 bp long fragment of PTS enzyme I gene from GAS, in channel 02 using a FAM-labelled probe and a 71 bp long fragment of phospholipase D gene from AH, in channel 03 using a HEX-labelled probe. The (+) Ctrl comprises plasmids with a 324 bp long synthetic fragment of PTS enzyme I gene for GAS and a 395 bp long synthetic fragment of phospholipase D gene for AH. (Accession number: CP000261 position 1148809 - 1149134 for GAS and L16583 position 264 - 469 and 677 - 867 for AH respectively). en UC-TIB-GAS-AH is an automated qualitative in vitro test for the detection of Streptococcus pyogenes (GAS) DNA from putative phosphoenolpyruvate sugar PTS enzyme I gene and Arcanobacterium haemolyticum (AH) DNA from phospholipase D gene. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of GAS and AH. en The limit of detection (LoD) for UC-TIB-GAS-AH was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 μL sample volume). The study demonstrates that the UC-TIB-GAS-AH detects at least 32 genome equivalent copies or less per mL for GAS and 18 copies or less per mL for AH, determined by LOGIT analysis with a hit rate of 95%. en

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