For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Gastro-Virus-2 SaV hPeV EntV AstV RUO UC-TIB-Gastro-Virus-2 (SaV, hPeV, EntV, AstV) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001251 10 761 458 001 10761458001 UC-TIB-Gastro-Virus-2 UC-TIB-Gastro-Virus-2 07613336243954 Reagents, kits 192 reactions Not Available false UC-TIB-Gastro-Virus-2 is an automated qualitative in vitro test for the detection of gastrointestinal viral RNA. The test utilizes amplification of target RNA by RT-PCR and nucleic acid hybridization for the detection of Sapovirus (SaV), human Parechovirus (hPeV), Enterovirus (EntV) and Astrovirus (AstV). en The limit of detection (LoD) for UC-TIB-Gastro-Virus-2 was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 µL sample volume). The study demonstrates that the UC-TIB- Gastro-Virus-2 detects at least 110 genome equivalent copies or less per mL for SaV, 170 copies or less per mL for hPeV, 150 copies or less per mL for EntV and 175 copies or less per mL for AstV, determined by LOGIT analysis with a hit rate of 95%. en Viral RNA is analyzed by real-time PCR that amplifies a 98 bp long fragment of the SaV RdRp gene, in channel 01 using Coumarin-labelled probes, a 74 bp long fragment of the hPeV 5´-UTR, in channel 02 using a FAM-labelled probe a 150 bp long fragment of the hPeV 5´-UTR in channel 03 using a HEX- labelled probe and a 106 bp long fragment of the viral capsid gene from Astrovirus in channel 04 using a LC640 labelled probe. The (+) Ctrl comprises plasmids with a 188 bp long synthetic fragment of SaV RdRp gene, a 342 bp long synthetic fragment of hPeV 5´-UTR, a 342 bp long synthetic fragment of EntV 5´-UTR and a 459 bp long synthetic fragment of AstV orf1b gene (Accession number: MG515479 position 5032 - 5219 for SaV RdRp gene, FM178558 position 415 - 746 for hPeV 5´-UTR, FM178558 position 415 - 746 for EntV 5´-UTR and KY744138 position 571 - 1029 for AstV orf1b gene respectively). en

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UC-TIB-Gastro-Virus-2 (SaV, hPeV, EntV, AstV)

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