For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-HFE RUO UC-TIB-HFE 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001082 10 602 040 001 10602040001 UC-TIB-HFE UC-TIB-HFE Reagents, kits 192 reactions Not Available false UC-TIB-HFE is an automated qualitative in vitro test designed to detect common mutations in the human HFE gene (OMIM: 235200). It uses RT-PCR for target DNA amplification and nucleic acid hybridization to identify the wild-type gene, along with the p.H63D (c.187C>G; rs1799945) and p.C282Y (c.845G>A; rs1800562) single nucleotide polymorphism variants. en This assay detects at least 1000 genome equivalent copies or less per mL (plasmid DNA dilution). LoD not tested. en Real-time PCR is employed to analyze human DNA for HFE gene mutations. To detect the p.C282Y (c.845G>A) polymorphism, a 103 bp fragment is amplified. The wild-type p.C282 (c.845G) is detected in channel 01 using a Coumarin-labeled probe, and the p.C282Y mutation (c.845G>A) is detected in channel 04 using an LC640-labeled probe. Concurrently, to detect the p.H63D (c.187C>G) polymorphism, a 93 bp fragment of the HFE gene is amplified. The wild-type p.H63 (c.187C) is detected in channel 02 using a FAM-labeled probe, and the p.H63D mutation (c.187C>G) is detected in channel 03 using a HEX-labeled probe.The positive controls include plasmids containing synthetic HFE gene fragments: a 524 bp wild-type (p.C282) fragment, a 524 bp mutated (p.C282Y) fragment (both from Accession number: AF525499, positions 63–586), a 373 bp wild-type (p.H63) fragment, and a 373 bp mutated (p.H63D) fragment (both from Accession number: U91328, positions 50249–49877). en

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