For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-HLA B27 RUO UC-TIB-HLA B27 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000862 10 316 489 001 10316489001 UC-TIB-HLA-B27 UC-TIB-HLA-B27 Reagents, kits 192 reactions Not Available false The Human Leukocyte Antigen (HLA) system is the major histocompatibility complex (MHC) in humans, consisting of many genes for cell-surface antigen-presenting proteins located on chromosome 6. HLA-B27 is encoded by the B locus, including subtypes B*27:01 to *27:280 (subject to extension from the time of this publication). en This assay detects at least 1000 genome equivalent copies or less per mL (plasmid DNA dilution). LoD not tested. More than 99.7% of the known human leukocyte antigen HLA-B27 gene variants are detected, exceptions are B*27:05:23, B*27:05:39, B*27:05:51, B*27:12:01:01, B*27:12:01:02, B*27:12:01:03, B*27:23, B*27:91, B*27:101, B*27:137, B*27:157, B*27:189, B*27:204:01:01, B*27:204:01:02, B*27:217, B*27:239, B*27:242, B*27:250. en Using PCR methodology, a 142 bp fragment of the HLA-B27 gene is amplified with specific primers and detected in channel 02 with a FAM-labeled probe. In addition a 193 bp fragment from the Glyceraldehyde 3- phosphate dehydrogenase gene is amplified as internal control and detected in channel 03 with a HEX- labeled probe.The (+) Ctrl comprise a plasmid with a 578 bp long synthetic fragment of HLA-B27 gene (B*27:02:01:04) in a background of human DNA (Accession number: MK279527 position 558 - 1135). en

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