For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-HPV-GT HPV31 33 45 52 58 RUO UC-TIB-HPV-GT (HPV31/33/45/52/58) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000860 10 316 497 001 10316497001 UC-TIB-HPV-GT UC-TIB-HPV-GT Reagents, kits 192 reactions Not Available false The limit of detection (LoD) for UC-TIB-HPV-GT was determined by analysis of serial dilutions of the WHO International Standards for HPV DNA genotypes HPV31, HPV33, HPV45, HPV52, HPV58 obtained from NIBSC (Code 19/226), in HPV-negative samples in Roche Cell Collection Medium (RCCM) (400 μL sample volume). The study demonstrates that the UC-TIB-HPV-GT detects HPV31 DNA at a concentration of 86 IU/mL, HPV33 DNA at a concentration of137 IU/mL, HPV45 DNA at a concentration of 87 IU/mLand HPV52/58 at a concentration of 235 IU/mL and 128 IU/mL respectively, determined by LOGIT analysis with a hit rate of 95%. en UC-TIB-HPV-GT is an automated qualitative in vitro test for the detection of human papillomavirus (HPV) DNA. The test utilizes amplification of target DNA by PCR and nucleic acid hybridization for the detection of 5 high-risk HPV types in a single analysis. The test specifically identifies HPV31, HPV33, HPV45 and HPV52/58. en HPV DNA is analyzed by real-time PCR that amplifies a 202 bp long fragment of L1 gene and differentiates HPV33 in channel 01 using a Coumarin-labelled probe, HPV52 and HPV58 in channel 02 using FAM-labelled probes, HPV45 in channel 03 using a HEX-labelled probe and HPV31 in channel 04 using a LC640 labelled probe. HPV52 and HPV58 are analyzed in the same channel and cannot be differentiated from each other. Other HPV genotypes like HPV16, HPV18, HPV35, HPV39, HPV51, HPV56, HPV59, HPV66 or HPV68 are not detected. en

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UC-TIB-HPV-GT (HPV31/33/45/52/58)

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