For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-MRSA RUO UC-TIB-MRSA 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001252 10 761 482 001 10761482001 UC-TIB-MRSA UC-TIB-MRSA 07613336243985 Reagents, kits 192 reactions Not Available false MRSA DNA is analyzed by real-time PCR that amplifies a 111 bp long fragment of the lukS PV gene (CA- MRSA), in channel 01 using a Cyan500 labelled probe, a 91 bp long fragment of the S.aureus nuc gene, in channel 02 using a FAM-labelled probe, a 185 - 304 bp long fragment of the orfX gene, in channel 03 using a HEX-labelled probe, a 134 bp long fragment of the mecA gene and a 137 bp long fragment of the mecC gene, in channel 04 using LC640 labelled probes respectively. For the verification of the presence of amplifiable nucleic acids, a 185 bp long fragment from the human Globin gene is amplified and detected with a Cy5.5 labeled probe in channel 05. The (+) Ctrl comprises plasmids with a 162 bp long synthetic fragment of lukS PV gene, a 320 bp long synthetic fragment of orfX gene, a 226 bp long synthetic fragment of nuc gene, a 228 bp long synthetic fragment of mecA gene and a 1668 bp long synthetic fragment og human globin gene (Accession number: AB186917 position 737 – 898 for lukS PV gene, BA000017 position 33975 - 34295 for orfX gene, CP094927 position 2714080 - 2714313 for nuc gene, PP449369 position 1664 - 1891 for mecA gene and L26463 positon 638 – 2308 for hu. globin gene respectively). en UC-TIB-MRSA is an automated qualitative in vitro test for the detection of methicillin-resistant Staphylococcus aureus (MRSA) and community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of MRSA including Panton-Valentine Leukocidin gene (lukS PV). en The limit of detection (LoD) for UC-TIB-MRSA was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 µL sample volume). The study demonstrates that the UC-TIB-MRSA detects at least 365 genome equivalent copies or less per mL for, determined by LOGIT analysis with a hit rate of 95%. en

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