For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-NTM RUO UC-TIB-NTM 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001311 10 855 304 001 10855304001 UC-TIB-NTM UC-TIB-NTM 04260159337211 Reagents, kits 192 reactions Not Available false UC-TIB-NTM is an automated qualitative in vitro test for the detection of bacterial DNA. The test utilizes amplification of mycobacterial DNA by RT-PCR and nucleic acid hybridization for the detection of Mycobacterium kansasii (M.kan), Mycobacterium avium complex (M.avi), Mycobacterium gordonae (M.gord) and Mycobacterium abscessus complex (M.abs). en The limit of detection (LoD) for UC-TIB-NTM was determined by analysis of serial dilutions of plasmid DNA in cobas® Microbial Inactiviation Solution (MIS, P/N 08185476001) (800 µL MIS added to 400 µL sample). The study demonstrates that the UC-TIB-NTM detects at least XX genome equivalent copies or less per mL for M.kan, XX copies or less per mL for M.avi, XX copies or less per mL for M.gord and XX copies or less per mL for M.abs, determined by LOGIT analysis with a hit rate of 95%. en Bacterial DNA is analyzed by real-time PCR that amplifies a 108 - 124 bp long fragment of mycobacterial ITS gene. M.kan is detected in channel 01 using a Coumarin-labelled probe, M.avi in channel 02 using a FAM-labelled probe, M.gord in channel 03 using a HEX-labelled probe and M.abs in channel 04 using a LC640 labelled probe.The (+) Ctrl comprises plasmids with a 370 bp long synthetic fragment of M.kan ITS gene, a 436 bp long synthetic fragment of M.avi ITS gene, a 347 bp long synthetic fragment of M.gord ITS gene and a 417 bp long synthetic fragment of M.abs ITS gene (Accession number: CP019886 position 1386644 - 1387013 for M.kan, EF521898 position 1 - 437 for M.avi, AB026692 position 75 - 422 for M.gord and DQ866776 position 1428 - 1844 for M.abs respectively). en

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