For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-PVL RUO UC-TIB-PVL 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00000857 10 356 847 001 10356847001 UC-TIB-PVL UC-TIB-PVL Reagents, kits 192 reactions Not Available false Community-associated Methicillin resistant Staphylococcus aureus (CA-MRSA) is a subclass of MRSA with an enhanced virulence in a number of species including (but not limited) to humans, domestic animals, pets and wild birds, caused by an extended production of phenol-soluble modulins. UC-TIB-PVL test is based on detection of the Panton-Valentine Leukocidin gene (lukS PV). en The limit of detection (LoD) for UC-TIB-PVL was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (200 μL sample volume). The study demonstrates that the UC-TIB-PVL detects at least 75 genome equivalent copies or less per mL, determined by LOGIT analysis with a hit rate of 95%. en CA-MRSA PVL DNA is analyzed by real-time PCR that amplifies a 111 bp long fragment of the lukS PV gene, in channel 01 using a Cyan500 labelled probe.The (+) Ctrl comprise a plasmid with a 162 bp long synthetic fragment of lokS PV gene (Accession number: AB186917 position 737 - 898). en

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