For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Respi-BAC-3 RUO UC-TIB-Respi-BAC-3 (M.pneu/L.pneu/C.pneu/C.psi) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001015 10 528 656 001 10528656001 UC-TIB-Respi-BAC-3 UC-TIB-Respi-BAC-3 Reagents, kits 192 reactions Not Available false The limit of detection (LoD) for UC-TIB-Respi-BAC-3 was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 μL sample volume). The study demonstrates that the UC-TIB-Respi-BAC-3 detects at least 66 genome equivalent copies or less per mL for L.pneu, 50 copies or less per mL for C.pneu, 46 copies or less per mL for M.pneu and 62 copies or less per mL for C.psi., determined by LOGIT analysis with a hit rate of 95%. en UC-TIB-Respi-BAC-3 is an automated qualitative in vitro test for the detection of bacterial DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Legionella pneumophila (L.pneu), Chlamydia pneumoniae (C.pneu), Mycoplasma pneumoniae (M.pneu) and Chlamydia psittaci (C.psi). en Bacterial DNA is analyzed by a RT-PCR that amplify a 78 bp long fragment of mip gene from L.pneu analyzed with a Coumarin-labeled probe in channel 01, a 79 bp long fragment of argR gene from C.pneu analyzed with a FAM-labeled probe in channel 02, a 98 bp long fragment of CARDS toxin gene from M.pneu analyzed with a HEX-labeled probe in channel 03 and a 92 bp long fragment of rpoB gene from C.psi analyzed with a LC640 labeled probe in channel 04. The closely related C. caviae and C. abortus may also be detected in channel 04, based on in-silico analysis. The positive control comprises plasmids with a 355 bp long synthetic fragment of L.pneu mip gene, a 392 bp long synthetic fragment of C.pneu argR gene, a 399 bp long synthetic fragment of M.pneu CARDS toxin gene and a 387 bp long synthetic fragment of C.psi rpoB gene (Accession number: AF023173 position 16 - 371 for L.pneu mip gene, LN849024 position 2711 - 3102 for C.pneu argR gene, DQ447750 position 1376 - 1763 for M.pneu CARDS toxin gene and AY826976 position 1932-2316 for C.psi rpoB gene respectively). en

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UC-TIB-Respi-BAC-3 (M.pneu/L.pneu/C.pneu/C.psi)

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