For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Respi-BAC-4 RUO UC-TIB-Respi-BAC-4 (B.pert/B.para/C.pneu/M.pneu) 100 1377683945183 This product is not a Roche product, Roche Global Customer Support is not offering any Support. For any additional information and support please ONLY contact TIB MolBiol directly. Furthermore we can not guarantee that all available information for this product is up to date. PID00001310 10 855 355 001 10855355001 UC-TIB-Respi Bac 4 UC-TIB-Respi Bac 4 04260159337297 Reagents, kits 192 reactions Not Available false This assay detects at least 100 genome equivalent copies or less per mL (plasmid DNA dilution).LoD not tested. en UC-TIB-Respi-Bac-4 is an automated qualitative in vitro test for the detection of respiratory bacterial DNA. The test utilizes amplification of target DNA by RT-PCR and nucleic acid hybridization for the detection of Bordetella pertussis / holmesii (B.pert), Bordetella parapertussis / bronchiseptica (B.para), Chlamydia pneumoniae (C.pneu) and Mycoplasma pneumoniae (M.pneu). en Bacterial DNA is analyzed by real-time PCR, that amplifies a 69 bp long fragment of IS481 gene from B.pert analyzed with a Coumarin-labeled probe in channel 01, a 79 bp long fragment of argR gene from C.pneu analyzed with a FAM-labeled probe in channel 02, a 80 bp long fragment of IS1001 gene from B.para analyzed with a HEX-labeled probe in channel 03 and a 98 bp long fragment of CARDS toxin gene from M.pneu analyzed with a LC640-labeled probe in channel 04.The (+) Ctrl comprises plasmids with a 363 bp long synthetic fragment of IS481 gene from B.pert, a 495 bp long synthetic fragment of IS1001 gene from B.para, a 392 bp long synthetic fragment of argR gene from C.pneu and a 399 bp long synthetic fragment of CARDS toxin gene from M.pneu (Accession number: M28220 position 695 - 1057 for IS481 gene from B.pert, X66858 position 1 - 495 for IS1001 gene from B.para, LN849024 position 2711 - 3102 for argR gene from C.pneu and DQ447750 position 1367 - 1763 for CARDS toxin gene from M.pneu respectively). en

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UC-TIB-Respi-BAC-4 (B.pert/B.para/C.pneu/M.pneu)

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