For Research Use Only. Not for use in diagnostic procedures. Others UC-TIB-Tickborne-3 RUO UC-TIB-Tickborne-3 (A.phago, Babesia, Ehrlichia, Borrelia) PID00001083 10 628 405 001 10628405001 UC-TIB-Tickborne-3 UC-TIB-Tickborne-3 Reagents, kits 192 reactions Not Available false The limit of detection (LoD) for UC-TIB-Tickborne-3 was determined by analysis of serial dilutions of plasmid DNA in cobas® PCR media (400 μL sample volume). The study demonstrates that the UC-TIB-Tickborne-3 detects at least 60 genome equivalent copies or less per mL for A. phago, 20 copies or less per mL for Babesia, 40 copies or less per mL for Ehrlichia and 70 copies or less per mL for Borrelia spp., determined by LOGIT analysis with a hit rate of 95%. en A. phago DNA is analyzed amplifying a 88 bp long fragment of the msp2 gene with a Coumarin-labeled probe in channel 01, for Babesia spp. a 93 bp long fragment of the 18S rRNA gene is amplified and detected with a FAM-labeled probe in channel 02, for Ehrlichia spp. a 101 bp long fragment of the 16S rRNA gene is amplified and detected with a HEX-labeled probe in channel 03 and for Borrelia spp. a 74 bp long fragment of the 23S rRNA gene is amplified and detected with a LC640 labeled probe in channel 04. The (+) Ctrl comprises plasmids with a 250 bp long synthetic fragment of msp2 gene of A. phago, a 188 bp long synthetic fragment of 18S rRNA gene of Babesia spp., a 560 bp long synthetic fragment of 16S rRNA gene of Ehrlichia spp. and a 403 bp long synthetic fragment of 23S rRNA gene of Borrelia spp. (Accession number: DQ519570 position 194 - 443 for msp2 gene of A. phago, AY789076 position 391 - 579 for 18S rRNA gene of Babesia spp., GQ501090 position 7 - 566 for 16S rRNA gene of Ehrlichia spp. and NR_076348 position 585 - 990 for 23S rRNA gene of Borrelia spp. respectively). en UC-TIB-Tickborne-3 is an automated qualitative in vitro test for the detection of Anaplasma phagocytophilum (A. phago), Babesia spp., Ehrlichia spp. and Borrelia spp. DNA. The Borrelia spp. detected, include Borrelia burgdorferi sensu stricto, B. miamotoi, B. afzelii, B. spielmanii, B. garinii, B. lusitaniae and others. en

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UC-TIB-Tickborne-3 (A.phago, Babesia, Ehrlichia, Borrelia)

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