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For in vitro diagnostic use. Others ultraView Universal Alkaline Phosphatase Red Detection Kit IVD ultraView Universal Alkaline Phosphatase Red Detection Kit RTD001184 05 269 814 001 5 269 814 001 05269814001 5269814001 05269814001 ULTRAVIEW UNIVERSAL AP RED DETECTION KIT ultraView Universal Alkaline Phosphatase Red Detection Kit 04015630972258 Reagents, kits 760-501 250 tests Not Available true The ultraView Universal Alkaline Phosphatase Red Detection Kit (ultraView AP Red detection kit) is an indirect biotin-free system for detecting mouse IgG, mouse IgM and rabbit primary antibodies using light microscopy. The kit is intended for laboratory use to identify targets by immunohistochemistry (IHC) in sections of formalin-fixed, paraffin-embedded tissue that are stained on BenchMark IHC/ISH instruments.
This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls.
This product is intended for in vitro diagnostic (IVD) use. en The ultraView AP Red detection kit detects specific mouse and rabbit antibodies bound to an antigen in formalin-fixed, paraffin-embedded (FFPE) tissue sections. The specific antibody is located by a cocktail of enzyme-labeled secondary antibodies that locate the bound primary antibody. The complex is then visualized with Naphthol substrate and Fast Red chromogen, which produces a red precipitate that is readily observed by light microscopy.The staining protocol consists of numerous steps in which reagents are incubated for predetermined times at specific temperatures. At the end of each incubation step, the BenchMark IHC/ISH instrument washes the sections to remove unbound material and applies a liquid coverslip which minimizes the evaporation of the aqueous reagents from the slide.1 Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.For more detailed information on instrument operation, refer to the appropriate User Guide.Figure 1 illustrates the indirect detection method.Figure 1. ultraView Universal Alkaline Phosphatase Red Detection Reaction.1. Elias JM, Gown AM, Nakamura RM, Wilbur DC, Herman GE, Jaffe ES, Battifora H, Brigati DJ. Quality control in immunohistochemistry. Report of a workshop sponsored by the Biological Stain Commission. Am J Clin Pathol. 1989;92(6):836- 843. en