KAPA Cleanup Beads

KAPA HyperPure Beads are optimized for use with different DNA and RNA library preparation research workflows:

• DNA Library Prep workflows, including KAPA EvoPlus V2 Kits and KAPA EvoPrep Kits
• RNA Library Prep workflows, including KAPA RNA EvoPrep Kits

• Compatible with KAPA DNA and RNA library preparation protocols
• Comparable yields and size distribution in comparison to industry-leading supplier
• Readily incorporated into existing automation applications

Figure 1: KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows.

Figure 1 shows recovery of unfragmented human gDNA. KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup.

Figure 2: KAPA HyperPure Beads provide superior recovery performance to Supplier A in DNA workflows.

Figure 2 shows recovery of highly degraded and moderately degraded formalin-fixed paraffin-embedded (FFPE) human genomic DNA. Highly degraded DNA obtained from FFPE clinical research samples (n=12); moderately degraded DNA obtained from Horizon Reference FFPE DNA (n=9). KAPA HyperPure Beads and Supplier A beads were used at a 3X ratio to clean up DNA inputs of various amounts. Recovery was measured using the Qubit Fluorometer 3 dsDNA HS Assay Kit before and after cleanup.

• Reproducible size selection of nucleic acids - capture of fragmented input DNA, adapter-ligated molecules, or amplified libraries in NGS library preparation workflows
• Enables researchers to consistently select for fragment sizes of interest without bias
• Fast, efficient reaction cleanups remove adapters, adapter-dimers, primers, primer-dimers, nucleotides, salts, and enzymes
• KAPA HyperPure Beads are ready-to-use and automation-friendly

Figure 3: KAPA HyperPure Beads provide tunable size selection of double-stranded DNA.

At each step of the library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit (Figure 3). Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto the Bioanalyzer. These results represent post-fragmentation size selection of input DNA. High-quality hgDNA was mechanically fragmented with a Covaris® E220 Focused Ultrasonicator using conditions optimized to yield a mode fragment length of 250 – 400 bp, then subjected to size selection.

Figure 4: KAPA HyperPure Beads provide tunable size selection of double-stranded DNA.

At each step of the library preparation workflow, DNA was subjected to double-sided size selection using different bead-to-sample ratios (see legend) and then analyzed for size distribution with a Bioanalyzer® 2100 High Sensitivity Kit. Each panel shows the resulting fragment size ranges compared to same-stage DNA that was not size-selected. DNA concentrations were normalized prior to loading onto the Bioanalyzer. Post-amplification size selection. Libraries were amplified and final libraries were then subjected to size selection.