Educational Resources for IHC, ISH and Multiplexing

Yes. Roche carries a broad line of ready-to-use research products. The DISCOVERY ULTRA is flexible to run any third party reagents as well. These reagents include but are not limited to blockers, primary antibodies, detection antibodies, and detection kits. Reagents can be manually applied or fully automated.

Yes. Roche currently does not have a ready to use kit for detecting cell death, but third party assays such as TUNEL can be automated on the DISCOVERY ULTRA.

Yes, however Roche does not offer directly labeled fluorescent probes. Researchers can obtain superior results using our broad library of ready-to-use DISCOVERY fluorescence kits.  These kits use Tyramide Signal Amplification (TSA) for superior signal, and can be used with DIG- and DNP-labeled probes. Additionally, key steps are incorporated in the DISCOVERY Universal Procedure Software to allow directly labeled probe hybridization.

Any tissue type or species can be run on the DISCOVERY ULTRA, as long as the appropriate antibodies are incorporated into the assay. Note that the Omnimap and Ultramap HRP-conjugated antibodies are available as single species to detect mouse, rabbit, rat, and goat antibodies for more flexibility in designing assays.

DISCOVERY Universal Procedure Software has multiple opportunities to add blocking steps. Key steps include Option steps and Antibody blocking steps. Note that the Option steps will allow blocking reagents to incubate then they will be rinsed off. Antibody Blocking step will incubate then the following antibody will co-incubate with the blocking reagent. The two application selections allow for maximum flexibility in incorporating the blocking reagents.

DISCOVERY HQ products use unique haptens for robust sensitivity. In addition to HQ detection, DISCOVERY amplification kits use both unique haptens and tyramide for enzymatic amplification. The amplification kits can boost signal while maintaining specificity. See DISCOVERY Detection for more details.

Yes. Ready-to-use reagents can be registered and used immediately. Third party reagents can be either applied manually or fully automated in a user fillable dispenser. Protocols with manual applications will beep when the reagents need to be applied, allowing the user to manually access the slide to manually pipette on reagents.

DISCOVERY Purple, DISCOVERY Yellow, and DISCOVERY Teal are the optimal chromogens for detecting co-localization. DISCOVERY Purple and DISCOVERY Yellow will result in a reddish/orange hue. DISCOVERY Purple and DISCOVERY Teal will result in an indigo blue hue. DISCOVERY Yellow and DISCOVERY Teal will result in a green hue.

The amount of chromogenic colors that can be combined depends on many variables, including the stability of epitopes being detected, and the compatibility of chromogens being used. Conditions for each target should be optimized individually, then combined and evaluated for equivalent performance.