AXELIOS 1 Platform

Be one of the first to see the future of high-throughput next-generation sequencing with AXELIOS 1, the platform powered by sequencing by expansion that's the first of its kind. Designed to overcome tradeoffs between speed, accuracy, and throughput, the platform separates chemistry from measurement in two systems: the synthesis instrument and the sequencing instrument with integrated high-performance compute that performs automated, accelerated analysis.

Together, the two systems deliver high performance, flexibility, and support for multiple NGS research workflows. This enables a broad range of batch and application needs with the flexibility to perform high-throughput sequencing at scale.

Get set to take your science even further. Now, let's explore the different stages of the workflow. Run planning and setup begin with AXELIOS 1 Platform Manager. Whether you're in the lab or at your office, this intuitive software interface lets you plan and monitor your sequencing runs with minimal touchpoints.

You can easily upload sample sheets, create run orders, and confirm workflow details. Use saved run configurations to maximize operational efficiency. Check device status for both the synthesis and sequencing instruments. Once your run is planned, the platform is ready when you are.

After library preparation, the AXELIOS 1 workflow starts at the synthesis system. Here, DNA libraries are synthesized into Xpandomers - surrogate molecules 50 times longer than the original DNA template. These are engineered to produce high-resolution signals during sequencing with minimal background noise.

Loading is simple and straightforward, with efficient, low-volume consumables. The color-coded bottle and reagent tubes make placement intuitive. The reagents and consumables are modular and can be used to perform synthesis for up to four independent library pools in parallel.

After the reagents and consumables are loaded, the instrument performs automated Xpandomer synthesis for the loaded library pools according to the selected synthesis protocol. You can choose from various optimized synthesis protocols to support a broad range of sequencing methods. Once synthesis is complete, the Xpandomers are ready for sequencing.

The sensor module is at the heart of AXELIOS 1. It's loaded and stored on the sequencing instrument where high-accuracy, nanopore-based, single-molecule sequencing is performed. The CMOS-based sensor module has over 8 million wells and measures the signal as Xpandomers transit through millions of biological pores in parallel, enabling high data rates and staggering throughput. You can reuse the sensor module and store it on-deck to automate preparation for subsequent runs.

Everything about the deck layout is designed to make it easy to use. Color-coded and barcoded consumables match their deck positions for rapid and accurate loading. Each sequencing run begins with an automated pre-run check, followed by a pre-sequencing process that includes cleaning of the sensor module, formation of new bilayer and pore insertion. This step is repeated for each run, ensuring optimal performance every time. You also have the option to configure runtimes and queue up to four independent runs to scale with your data and batching needs.

As sequencing begins, the platform's integrated compute kicks in. In the SBX-D workflow, basecalling, demultiplexing, intramolecular consensus, and reference-based alignment all occur in real-time as data is generated. You don't have to wait for the run to finish for downstream analysis to begin. AXELIOS 1 is capable of generating industry-standard file types, including FASTQs and BAMs, all while the run is in progress.

For bioinformaticians looking for more control and access to secondary analysis tools optimized for SBX, our standalone XOOS analysis tools are readily available and customizable to fit your application and workflow needs when used with AXELIOS 1 data. These tools can be deployed on cloud or local compute infrastructure, giving you flexibility and control over your analysis workflows to realize the full potential of your sequencing.

AXELIOS 1 is a transformative platform, delivering accurate next-generation sequencing at groundbreaking scale and speed. Get set to be among the first to sequence in hours instead of days, and be one step ahead with AXELIOS 1.