1 platform. A world of possibilities.
The AXELIOS 1 is a transformative next-generation sequencing platform designed to bridge the gap between massive throughput and operational flexibility. Powered by novel sequencing by expansion (SBX) technology, the AXELIOS 1 Platform overcomes the signal-to-noise limitations of standard nanopores by converting target nucleic acids into easily measurable Xpandomer polymers. This ensures single-molecule accuracy without sacrificing the speed your most urgent projects demand.
The platform features a modular workflow designed to maximize flexibility while reducing operational costs. Following upstream library preparation, the synthesis instrument performs Xpandomer synthesis on up to four library pools in parallel. For total workflow autonomy, these pools can then be sequenced individually or queued for automated, sequential processing on the sequencing instrument with no manual intervention. By pairing this walkaway flexibility with cost-effective, reusable sensor module and integrated real-time data analysis, the AXELIOS 1 Platform accelerates your path from sample to data.
Explore AXELIOS 1 Platform. The 1st of its kind.
With a novel combination of SBX chemistry, ultra-fast and accurate single-molecule sequencing, and accelerated analysis, the AXELIOS 1 Platform has the potential to redefine the next-generation sequencing space with:
Flexibility: Optimized workflows and longer read lengths for diverse use cases; explore beyond the limitations of short reads.*
Speed: Rapid sequencing and near real-time data processing reduce time-to-data from days to hours.
Scalability: Tunable sequencing output and batch sizes support seamless scalability, from pilot to production-scale projects.
Accuracy: Achieve the level of accuracy desired for a wide range of methods and applications, all at a rapid pace.
Platform workflow
Library preparation
Library preparation may be performed manually or using automated liquid handling systems. While the same reagents support both simplex (SBX-S) and duplex (SBX-D) sequencing, the specific library prep workflow and application-specific steps will depend on the chosen mode.
Xpandomer synthesis
Perform synthesis for 1 to 4 independent library pools in parallel. The automated on-chip workflow hybridizes upstream DNA libraries to the surface and enzymatically transcribes them into Xpandomers via the incorporation of base-specific X-NTPs. The Xpandomer backbone is then chemically expanded, and the molecules are UV-cleaved from the surface. Finally, the sequencing-ready pools are eluted directly into barcoded output tubes that can be loaded onto the sequencing instrument.
Sequencing
The reusable sensor module utilizes a dense, CMOS-based architecture featuring millions of independent microwells to deliver massive throughput for Xpandomer sequencing. Engineered for multi-run durability, a single sensor module supports up to 20 independent sequencing runs.
Every automated run initiates with a specialized workflow consisting of bilayer formation and nanopore insertion on the sensor module. Once sequencing is complete, an integrated cleaning protocol thoroughly resets the module, preparing the surface for the next run and ensuring long-term reliability.
Analysis
Output files from on-instrument primary/post-primary analysis are compatible with standalone, customizable XOOS analysis tools (specifically developed and optimized for SBX data), as well as with third-party analysis tools.
SBX technology resources
SBX transforms tightly packed DNA into a longer molecule with distinct, high-signal markers. This allows rapid, highly accurate sequencing that can flexibly scale to support a wide range of applications. Watch the video to learn more!
SBX transforms tightly packed DNA into a longer molecule with distinct, high-signal markers. This allows rapid, highly accurate sequencing that can flexibly scale to support a wide range of applications. Watch the video to learn more!
Enter an entirely new space in NGS - watch this webinar to hear how our novel sequencing technology could expand your science and help you uncover richer insights from every sequence.
Enter an entirely new space in NGS - watch this webinar to hear how our novel sequencing technology could expand your science and help you uncover richer insights from every sequence.
Watch a high-level comparison between sequencing by expansion (SBX) and sequencing by synthesis (SBS) in simple terms. Using a printer analogy and a "spot the difference" game, this video illustrates how each method generates sequencing reads.
Watch a high-level comparison between sequencing by expansion (SBX) and sequencing by synthesis (SBS) in simple terms. Using a printer analogy and a "spot the difference" game, this video illustrates how each method generates sequencing reads.
Watch the recording to gain a better understanding of the methods used to generate SBX-D consensus reads, germline small variant calling and more.
Watch the recording to gain a better understanding of the methods used to generate SBX-D consensus reads, germline small variant calling and more.
Download this white paper to learn more about SBX: Sequencing By Expansion (SBX): A Fast, Flexible, and Accurate Single-molecule Sequencing Technology
Download this white paper to learn more about SBX: Sequencing By Expansion (SBX): A Fast, Flexible, and Accurate Single-molecule Sequencing Technology
Download this white paper to learn more about SBX: Germline variant calling: SBX with DeepVariant
Download this white paper to learn more about SBX: Germline variant calling: SBX with DeepVariant
Featured products
Dive deeper into SBX and NGS
AGBT 2026 workshop presentations
The Power of One: AXELIOS 1
Johanna Whitacre
Network Lead, Sequencing Research and Development
Roche Diagnostics Solutions
The Power of One: AXELIOS 1
Mark Kokoris
Head, SBX Technology
Roche Diagnostics Solutions
The Power of One: AXELIOS 1
Gustav Karlberg
Lifecycle Leader, Sequencing Systems
Roche Diagnostics Solutions
The Power of One: AXELIOS 1
Mitu Chaudhary
International Business Leader, Sequencing Systems
Roche Diagnostics Solutions
ABRF Technology Showcase presentation
Innovation in Sequencing: AXELIOS 1 and SBX technology for streamlining workflows in core facilities and beyond
Eric Chow, PhD
Director, Center for Advanced Technology (CAT)
UCSF
AACR exhibitor spotlight presentation
Whole Genome Sequencing (WGS) on AXELIOS 1: Detecting Low-Levels of ctDNA with High Accuracy
Alex Robertson
Director, Computational Biology
Foundation Medicine, Inc.
Workshop video:
Advances in Sequencing by expansion (SBX)
Multiomics, methylation mapping, oncology research and building a sustainable framework for ultra-rapid genome sequencing
Additional Presentations
Overview of AXELIOS Sequencing System and Research Applications in WGS
Mitu Chaundry
International business leader, SBX Technology
Roche Diagnostics Solutions
Jagdeesh Chandrasekar
Director of Advanced SBX applications, SBX Technology
Roche Diagnostics Solutions
Characterization of Cancer Transcriptomes and Fusion Isoforms using Roche SBX Technology
Brian J, Haas, Ph.D.
Principal Computational Scientist
Methods Development Laboratory (MDL)
Broad Institute, USA
SBX Technology for Single Cell R and Spatial Analysis
Yutaka Suzuki, Ph.D.
Professor
Life Science Data Research Center
Graduate School of Frontier Sciences
The University of Tokyo, Japan
Leveraging Extremely High Throughput and Longer Reads Capability of SBX Technology for Advancing Transcriptomics and Spatial Analysis
Emma Davenport
Group Leader
Wellcome Sanger Institute, UK
Download all event posters for ASHG 2025
Download all the event posters covering the following topics:
- Demonstrating the Versatility, Accuracy and Throughput of Sequencing By Expansion (SBX)
- Enhanced Detection of Structural Variants, VNTRs, and Haplotype Phasing with SBX Simplex Longer Sequencing (SBX-SL)
- Whole Genome Precision at Scale: Sequencing by Expansion for Cancer Genomics Research
- Whole Genome Sequencing Minimum Residual Disease Detection using Sequencing By Expansion (SBX)
Sequencing by Expansion (SBX): A versatile, high throughput single molecule sequencing technology
Mark Kokoris
Head, SBX Technology
Roche Diagnostics Solutions
Refining the SBX Fast workflow for reproducible speed and performance
Sean Hofherr, PhD, FACMG
Chief of Clinical Strategy and Product Development
Broad Clinical Labs
Redefining nanopore sequencing with Duplex Sequencing by Expansion (SBX-D)
A high-accuracy, high-throughput sequencing technology
SBX sequencing as a flexible RNA-seq toolkit
Enabling high throughput single cell transcriptomic profiling at scale
AGBT 2025 workshop presentations
SBX Technology
Mark Kokoris
Head, SBX Technology
Roche Diagnostics Solutions
Cancer Whole Genome Sequencing using SBX technology
Edwin Cuppen
Scientific director
Hartwig Medical Foundation
Enabling Rapid WGS for Trios by Roche SBX Technology
Sean Hofherr, PhD, FACMG
Chief of Clinical Strategy and Product Development
Broad Clinical Labs
Multiomic drug response profiling in highly multiplexed cancer cell lines
Aziz Al'Khafaji, PhD
Director, Molecular R&D
PI, Methods Development Lab
Broad Clinical Labs
AXELIOS 1* data analysis
*The technology is capable of generating longer reads of up to ~1500bp (with a distribution from ~200bp to ~1500 bp) under appropriate sample and library prep conditions.
For Research Use Only. Not for use in diagnostic procedures. AXELIOS is a trademark of Roche.
F. Hoffmann-La Roche Ltd. Data on File.