Prostate cancer IHC portfolio

Prostate cancer diagnostic solutions

Prostate cancer is a major public health concern. Prostate cancer is the most commonly diagnosed and second leading cause of cancer deaths in U.S. men.1 Approximately 1 man in 8 will be diagnosed with prostate cancer in his lifetime.2 Approximately 76% of prostate cancer cases are diagnosed in early stage. The 5 year survival rate for all stages is 97.8%.2

We offer a wide menu of immunohistochemistry (IHC) assays. Our portfolio of products delivers the high sensitivity and specificity you need.

Our antibodies are ready to use on the fully automated VENTANA BenchMark IHC/ISH staining platforms, reducing the time-to-result and resources required with manual or semi-automated solutions. We have a robust assay development program focusing on immunohistochemical staining, clinical reagents, cancer diagnostic assays, and much more.

Featured Assays

immunohistochemical staining with Basal Cell Cocktail 34ßE12 + p63
VENTANA PTEN (SP218) Rabbit Monoclonal Primary Antibody


The VENTANA PTEN (SP218) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the qualitative detection of the PTEN protein in formalin-fixed, paraffin-embedded tissue. It is intended to be stained with the VENTANA BenchMark ULTRA immunohistochemical automated slide stainer.  The clinical interpretation of any staining, or the absence of staining, must be complemented by histological studies and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests. This antibody is intended for in vitro diagnostic (IVD) use.

immunohistochemical staining with Basal Cell Cocktail 34ßE12 + p63
Basal Cell Cocktail (34ßE12 + p63), Ventana


VENTANA Basal Cell Cocktail (34βE12+p63) is an antibody cocktail of p63 (4A4) and keratin (34βE12). It may be used to aid in the differentiation of benign lesions with basal cells from malignant prostate lesions lacking basal cells. For more than 15 years, basal cells have been the immunohistochemical cornerstone in the diagnosis of prostate cancer3,4

Immunohistochemical staining of p504s (SP116) Rabbit Monoclonal Primary Antibody
p504s (SP116) Rabbit Monoclonal Primary Antibody


p504s (SP116) Rabbit Monoclonal Primary Antibody is directed against the p504s enzyme (also known as alpha-Methylacyl Coenzyme A racemase or AMACR). p504s is found to be overexpressed in human prostatic carcinoma, and exhibits a granular, cytoplasmic staining pattern in malignant glands and cells.12

Publications demonstrate the utility of p504s as an aid to the pathologist in recognizing small foci prostatic carcinoma when used to complement an absence of basal cell staining.13 This can be achieved in a dual stain with the Basal Cell Cocktail (34βE12 + p63) to optimize the use of limited available tissue on small focal cancer biopsies.14

ihc detection: NKX3.1 (EP356) Rabbit Monoclonal Primary Antibody
NKX3.1 (EP356) Rabbit Monoclonal Primary Antibody


NKX3.1 (EP356) Rabbit Monoclonal Primary Antibody is a sensitive and specific antibody for the detection of NKX3.1 protein. Publications demonstrate that the reliable detection of NKX3.1 may have utility in a number of clinical applications in prostatic carcinoma and breast carcinomas.8

  • Prostatic carcinoma: There is a high frequency of NKX3.1 expression in prostatic adenocarcinoma with greater than 98% sensitivity and specificity.9,10
  • Gurel et al., demonstrated that nearly all cases of metastatic prostate adenocarcinoma were stained by NKX3.1.10
  • Gurel and Chuang showed that nearly all cases of urothelial carcinoma were negative for NKX3.1.10,11
  • NKX3.1-positive prostate carcinoma cells exhibit nuclear staining.10
Clinical reagents and assays include the p63 (4A4) Mouse Monoclonal Primary Antibody
p63 (4A4) Mouse Monoclonal Primary Antibody, VENTANA


The VENTANA p63 antibody is directed against the p63 molecule, which is highly expressed in the nuclei of human prostatic basal cells and urothelial tissues. This powerful tool can aid the pathologist in the differential diagnosis of prostate cancer in conjunction with morphological findings. Delivering consistently strong nuclear staining, the VENTANA p63 antibody may be used to aid in the differentiation of benign and malignant prostatic lesions.15,16

Clinical reagents and assays include the p63 (4A4) Mouse Monoclonal Primary Antibody
anti-ERG (EPR3864) Rabbit Monoclonal Primary Antibody


The VENTANA ERG (EPR3864) Rabbit Monoclonal Primary Antibody is capable of detecting:

  • Truncated ERG resulting from TMPRSS2:ERG (or other ERG gene fusions)
  • Wild type ERG (most notably expressed in vessel endothelium)

Antibody Research has demonstrated that the TMPRSS2:ERG rearrangement occurs in approximately 50% of prostate cancer patients, does not occur in normal tissue, and describes a molecular subtype that is associated with androgen-driven prostate cancer. As this subtype is the most prevalent in prostate cancer, there has been tremendous diagnostic, prognostic and predictive interest in the ERG biomarker. 5,6,7

Resources  / 

  1. NCCN Clinical Practice Guidelines in Oncology, Prostate Cancer Early Detection Version 1.2020 – July 24, 2020
  2. Accessed 12/01/2020
  3. Brawer MK, Peehl DM, Stamey TA, Bostwick DG. Keratin immunoreactivity in the benign and neoplastic human prostate. Cancer Res1985; 45(8):3663-7.
  4. Hedrick L, Epstein JI. Use of keratin 903 as an adjunct in the diagnosis of prostate carcinoma. Am J Surg Pathol 1989; 13(5):389-96. 
  5. Shah R B, et al. The diagnostic use of ERG in resolving an “atypical glands suspicious for cancer” diagnosis in prostate biopsies beyond that provided by basal cell and α-methylacyl-CoAracemase markers. Human pathology. 2013; 44(5): 786 -794.
  6. Weischenfeldt et al. Integrative Genomic Analyses Reveal an Androgen-Driven Somatic Alteration Landscape in Early-Onset Prostate Cancer. Cancer Cell. 2013; 23:159-170.
  7. Park, K et al. Antibody-based detection of ERG rearrangement-positive prostate cancer. Neoplasia. 2010; 12(7): 590.
  8. Asch-Kendrick R, et al. NKX3.1 is expressed in ER-positive and AR-positive primary breast carcinomas. J Clin Pathol. 2014; 67:768-71. 
  9. NKX3.1 (EP356) Package Insert
  10. Gurel B, et al. NKX3.1 as a marker of prostatic origin in metastatic tumors. Am J Surg Pathol. 2010; 34:1097-1105.
  11. Chuang A, et al. Immunohistochemical differentiation of high-grade prostate carcinoma from urothelial carcinoma. Am J Surg Pathol. 2007; 31:1246-55.
  12. Jiang J et al. Using an AMACR (P504S/34βE12/p63 Cocktail for the Detection of Small Focal Prostate Carcinoma in Needle Biopsy Specimens. Am J Clin Pathol. 2005; 123:231-236.
  13. Epstein, J I. Diagnosis of limited adenocarcinoma of the prostate. Histopathology. 2012; 60:28-40.
  14. Ng, V W et al. Is Triple Immunostaining With 34βE12, p63, and Racemase in Prostate Cancer Advantageous? A Tissue Microarray Study. Am J Clin Pathol. 2007; 127:248-253. 
  15. Weinstein MH, et al. Diagnostic utility of immunohistochemical staining for p63, a sensitive marker of prostatic basal cells. Mod Pathol 2002; 15:1302-1308.
  16. Parsons et al. p63 protein expression is rare in prostate adenocarcinoma: implications for cancer diagnosis and carcinogenesis. Urology. 2001; 58:619-24.


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