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Key Takeaways
- SBX performance data: Review experimental designs and datasets from two workflows demonstrating sequencing-by-expansion (SBX) performance on the AXELIOS 1 platform for WGS of both ctDNA and T/N samples.
- Scale for MRD research: Learn how ultra-high throughput sequencing provides the necessary depth of coverage to confidently detect extremely low tumor fractions and faint molecule signatures in ctDNA.
- Overcome FFPE sample hurdles: Learn how to establish a robust somatic variant baseline from degraded FFPE T/N tissue pairs.
- Advanced analysis: Discover how the free, SBX-optimized open-source (XOOS) analysis toolkit powers these workflows to deliver benchmarked accuracy and speed for SBX somatic variant calling.
Webinar overview
In minimal residual disease (MRD) research, identifying faint molecular signatures demands extreme analytical sensitivity to overcome low input abundance and background noise. While matched FFPE tumor/normal (T/N) sequencing helps resolve true somatic mutations from germline variants, solid tissue profiling presents its own hurdles, including sample degradation and fixation-induced sequencing artifacts.
This webinar demonstrates a unified, tumor-informed workflow that establishes a robust somatic variant baseline from degraded FFPE T/N tissue pairs while leveraging high-throughput sequencing for ultra-sensitive ctDNA detection in MRD research.
Meet the speakers
Grete Sittmann
International Product Manager, Sample Prep Reagents
Roche Sequencing Solutions
Grete Sittmann is an international product manager for sample prep reagents at Roche Sequencing Solutions, bringing over a decade of NGS experience to the team since joining Roche in 2015. Previously a principal scientist leading R&D teams in Cape Town, South Africa, she managed the development and launch of foundational KAPA Sample Prep Reagents offerings. Now based in Pleasanton, CA, she formed part of the team that launched the AXELIOS 1 Platform (representing the ecosystem for library prep reagents) and continues to champion high-impact application notes and workflows.
Mahdi Golkaram
Director of Bioinformatics
Roche Sequencing Solutions
Mahdi Golkaram is the Director of Bioinformatics Data Science at Roche, where he leads algorithm development for the secondary analysis of Sequencing by Expansion (SBX) data. He played a key role in the successful development and launch of SBX, a next-generation sequencing technology, and led the creation of XOOS—a free and open-source bioinformatics analysis suite tailored for SBX.
By integrating cutting-edge bioinformatics algorithms and data-driven approaches, including deep learning and artificial intelligence, XOOS enables SBX users to detect genetic alterations from sequencing data to support a wide range of research applications. These span genetic disease, oncology, and infectious disease research, through multi-omics whole genome and targeted sequencing with both SBX simplex and duplex technologies.
For Research Use Only. Not for use in diagnostic procedures. AXELIOS is a trademark of Roche.
