LightCycler® PRO System

Light the way from research to clinical diagnostics with the next evolution in the Roche qPCR portfolio

IVD For in vitro diagnostic use.
LightCycler® PRO System
A qPCR system designed to deliver accuracy and flexibility to research and clinical diagnostic laboratories

Leveraging the robustness and proven technology of the LightCycler® 4801, the  LightCycler® PRO integrates innovative technology and Roche manufacturing to deliver accurate real-time PCR results, flexible solutions to your workflow, and an improved user experience to your lab.

LightCycler Pro System
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Meet the newest addition to the Roche real-time PCR portfolio


To give you precise and reproducible results


To support customization and standardization of workflows based on your lab’s unique needs

Enhanced user-experience

To optimize your operational efficiency
Explore the newest qPCR analyzer for your lab's research and clinical diagnostic needs
lightcycler pro expanded
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Have confidence in the accuracy of your results
  • Patented Vapor Chamber Mount design delivers precise temperature regulation and enhanced sensitivity.
  • Optical module design for uniform excitation, edge effect prevention, simultaneous data capture, and flexible dye options and detection formats.
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Adapt quickly to evolving testing needs
  • New 7-channel multiplexing increases hourly throughput and enables greater diversity of tests per run
  • Thermal Cycler with interchangeable 96- or 384-well plates offers variable throughput options to support your lab’s specific needs
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Optimize workflow efficiency with easy data management options
  • Large, 15.7 in touchscreen improves user interface and enables easy access to workflow and data management options 
  • New software for reliable and reproducible data analysis, easy import/export of data, LIS connectivity, and remote access
Improved algorithms deliver reliable data you can depend on

The new LightCycler® PRO software optimizes existing algorithms to improve functionality, performance, and precise data analysis.2

These new enhancements include:

  • A new adaptive algorithm which improves color compensation across the 7 channels to prevent crosstalk and contaminated results. This algorithm is optimized for use with LightMix® modular assays.
  • An updated derivative algorithm which automatically determines the Cq value from the PCR run data for confidence in results calling.
TIB Molbiol LightMix Modular Gastro Bacteria 7-target multiplexed assay

TIB Molbiol LightMix® Modular Gastro Bacteria 7-target multiplexed assay – optimized to detect 7 different targets from a single well

TIB Molbiol LightMix Modular Gastro Bacteria 7-target multiplexed assay

LightCycler® PRO System, dilution series 10^1 to 10^8 copies, demonstrating high precision and accuracy.


  1. For research use only. Not for use in diagnostic procedures.
  2. Data on file with Roche. V&V Report: DH-02714.04-500B.


Ordering Information

Technical Documents

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