Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Cytoplasm Others Calponin-1 EP798Y IVD Appendix Calponin-1 (EP798Y) Rabbit Monoclonal Antibody RTD000713 Rabbit EP798Y 05 435 684 001 5 435 684 001 05435684001 5435684001 05435684001 Calponin-1 (EP798Y) PAb, Cell Marque Calponin-1 (EP798Y) 00841683100744 Reagents, kits 50 tests Not Available true Calponin-1 (EP798Y) Rabbit Monoclonal Primary Antibody is intended for laboratory use in the detection of the Calponin-1 protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en Calponin-1 (EP798Y) Rabbit Monoclonal Antibody (this antibody) may be used as theprimary antibody for immunohistochemical staining of formalin-fixed, paraffin-embeddedtissue sections. In general, immunohistochemical staining allows the visualization of antigensvia the sequential application of a specific antibody (primary antibody) to the antigen, asecondary antibody (link antibody) to the primary antibody, an enzyme complex and achromogenic substrate with interposed washing steps. The enzymatic activation of thechromogen results in a visible reaction product at the antigen site. The specimen may then becounterstained and a coverslip applied. Results are interpreted using a light microscope andaid in the differential diagnosis of pathophysiological processes, which may or may not beassociated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits andBenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section forrecommended staining protocols. Each step in the staining protocol includes incubation fora precise time at a specifi c temperature. At the end of each incubation step, the sections arerinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unboundmaterial that would hinder the desired reaction in subsequent steps. To minimize evaporationof the aqueous reagents from the specimen-containing slide, a coverslip solution is appliedin the slide stainer. For more detailed information on instrument operation, refer to theappropriate BenchMark IHC/ISH instruments Operator’s Manual. en

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