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Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Membrane Others CD21 2G9 IVD Lymph Node Tonsil CD21 (2G9) Mouse Monoclonal Antibody RTD000723 Mouse 2G9 05 269 059 001 5 269 059 001 05269059001 5269059001 05269059001 Cell Marque, CD21 (2G9) CD21 (2G9) 00841683100461 Reagents, kits 50 tests Not Available true CD21 (2G9) Mouse Monoclonal Primary Antibody is intended for laboratory use in the detection of the CD21 protein in formalin-fixed, paraffin-embedded human tissue stained in qualitative immunohistochemistry (IHC) on BenchMark IHC/ISH instruments. This product should be interpreted by a qualified pathologist in conjunction with histological examination, relevant clinical information, and proper controls. This antibody is intended for in vitro diagnostic (IVD) use. en CD21 (2G9) Mouse Monoclonal Antibody (this antibody) may be used as the primary
antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue
sections. In general, immunohistochemical staining allows the visualization of antigens
via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a
chromogenic substrate with interposed washing steps. The enzymatic activation of the
chromogen results in a visible reaction product at the antigen site. The specimen may then be
counterstained and a coverslip applied. Results are interpreted using a light microscope and
aid in the differential diagnosis of pathophysiological processes, which may or may not be
associated with a particular antigen.
This antibody is optimally diluted to be compatible with VENTANA detection kits and
BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for
recommended staining protocols. Each step in the staining protocol includes incubation for
a precise time at a specific temperature. At the end of each incubation step, the sections are
rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound
material that would hinder the desired reaction in subsequent steps. To minimize evaporation
of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied
in the slide stainer. For more detailed information on instrument operation, refer to the
appropriate BenchMark IHC/ISH instruments Operator’s Manual. en
