For Research Use Only. Not for use in diagnostic procedures. Others KAPA EvoAmp ReadyMix RUO KAPA EvoAmp ReadyMix PID00001156 10 613 572 001 10613572001 KAPA EvoAmp RM pwd by KAPA HiFi (96rxn) KAPA EvoAmp RM pwd by KAPA HiFi (96rxn) 07613336239643 Reagents, kits 96 reactions Not Available 10 613 556 001 10613556001 KAPA EvoAmp RM pwd by KAPA HiFi (24rxn) KAPA EvoAmp RM pwd by KAPA HiFi (24rxn) 07613336239636 Reagents, kits 24 reactions Not Available 10 613 599 001 10613599001 KAPA EvoAmp RM pwd by KAPA HiFi (384rxn) KAPA EvoAmp RM pwd by KAPA HiFi (384rxn) 07613336239650 Reagents, kits 384 reactions Not Available KAPA EvoAmp ReadyMix contains KAPA HiFi HotStart DNA Polymerase that is specifically designed to minimize amplification bias, while maintaining extremely high fidelity. The KAPA EvoAmp ReadyMix is designed for efficient library amplification of adapter-ligated libraries for next-generation sequencing (NGS). KAPA EvoAmp ReadyMix is compatible with libraries constructed from input DNA amounts of 0.1 ng to 500 ng (high quality DNA). It is compatible with high-quality gDNA, cfDNA and low-quality DNA such as that extracted from formalin-fixed, paraffinembedded tissue (FFPET) samples. The KAPA EvoAmp ReadyMix Kits are designed for the amplification of next-generation sequencing (NGS) libraries prepared for Illumina sequencing. The KAPA EvoAmp ReadyMix Kits are ideally suited for high-efficiency, high fidelity, low-bias amplification of libraries prior to Illumina sequencing.This includes libraries prepared for Whole-genome sequencing (WGS) and Whole exome sequencing (WES) or targeted sequencing. KAPA EvoAmp ReadyMix Kits which are powered by KAPA HiFi HotStart build on the existing performance of KAPA HiFi which is ideally suited for high-efficiency, high fidelity, low-bias amplification of libraries prior to Illumina sequencing. This includes libraries prepared for: whole-genome shotgun sequencing exome or targeted sequencing (pre- and post-capture amplification) RNA-seq ChIP-seq other sequencing applications In order to maximize sequence coverage uniformity, it is critical to minimize library amplification bias. Amplification bias occurs when a DNA polymerase is unable to amplify all targets within a complex population of library DNA with equal efficiency. KAPA HiFi DNA Polymerase is a B-family DNA polymerase engineered for increased processivity, extremely high fidelity and low-bias, and is the reagent of choice for NGS library amplification.1,2,3,4 KAPA HiFi HotStart DNA Polymerase has 5’→3’ polymerase and 3’→5’ exonuclease (proofreading) activities. The error rate of KAPA HiFi HotStart DNA Polymerase is 2.8 x 10-7 errors/ base, equivalent to 1 error per 3.5 x 106 nucleotides incorporated. The enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This prevents nonspecific amplification during reaction setup, increases sensitivity, and improves reaction efficiency. KAPA EvoAmp ReadyMix, a ready-to-use Library Amplification mix comprising all the components for library amplification; except primers and template. 1. Oyola, S.O., et al., BMC Genomics 13, 1 (2012). 2. Quail, M.A., et al., Nature Methods 9, 10 (2012). 3. Quail, M.A., et al., BMC Genomics 13, 341 (2012). 4. Ross, M.G., et al., Genome Biology 14, R51 (2013). en

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