For Research Use Only. Not for use in diagnostic procedures. Others KAPA Library Quantification Kit RUO KAPA Library Quantification Kit SEQ-KAPA-0115 07 960 204 001 7 960 204 001 07960204001 7960204001 07960204001 Lib Quant Kit (ABI) Lib Quant Kit (ABI) 07613336112724 Reagents, kits KK4835 500 reactions Not Available 07 960 140 001 7 960 140 001 07960140001 7960140001 07960140001 Lib Quant Kit (Uni) Lib Quant Kit (Uni) 07613336112670 Reagents, kits KK4824 500 reactions Not Available 07 960 255 001 7 960 255 001 07960255001 7960255001 07960255001 Lib Quant Kit (BioRad) Lib Quant Kit (BioRad) 07613336112762 Reagents, kits KK4844 500 reactions Not Available 07 960 336 001 7 960 336 001 07960336001 7960336001 07960336001 Lib Quant Kit (ROX Low) Lib Quant Kit (ROX Low) 07613336112847 Reagents, kits KK4873 500 reactions Not Available 07 960 298 001 7 960 298 001 07960298001 7960298001 07960298001 Lib Quant Kit (LC480) Lib Quant Kit (LC480) 07613336112809 Reagents, kits KK4854 500 reactions Not Available KAPA Library Quantification Kits provide all the reagents needed for absolute, qPCR-based quantification of Illumina libraries flanked by the P5 and P7 flow cell oligo sequences. Kits contain: Library Quantification DNA Standards 1 – 6 (a 10-fold dilution series of a linear, 452 bp template) Library Quantification Primer Premix (10X), containing the following primers: Primer 1: 5'-AAT GAT ACG GCG ACC ACC GA-3' Primer 2: 5'-CAA GCA GAA GAC GGC ATA CGA-3' KAPA SYBR® FAST qPCR Master Mix (2X), available with various passive reference dyes. Library quantification is performed by amplifying the set of six pre-diluted DNA Standards and diluted library samples by qPCR, using the KAPA SYBR FAST qPCR Master Mix and primers targeting the Illumina P5 and P7 flow cell oligo sequences. The average Cq score for each DNA Standard is plotted against log10 (concentration in pM) to generate a standard curve. The concentrations of diluted library samples are then calculated against the standard curve, using absolute quantification. KAPA Library Quantification Kits are rigorously tested to ensure minimal lot-to-lot variation. The kit contains the novel KAPA SYBR FAST DNA Polymerase, engineered through a process of directed evolution for highperformance SYBR Green I-based qPCR. The ability of the engineered polymerase to amplify diverse DNA fragments with similar efficiency enables the use of a universal standard for the reliable quantification of all Illumina libraries with an average fragment length of up to 1 kb, irrespective of library type or GC content. KAPA SYBR FAST is an antibody-mediated hot start DNA polymerase formulation. KAPA Library Quantification Kits are therefore suitable for use with automated liquid handling systems for high-throughput sample quantification. en KAPA Library Quantification Kits are designed for the accurate and reproducible quantification of libraries prepared for Illumina sequencing. Any library with a concentration >0.0002 pM that contains sequences complementary to the primers in the Primer Premix (10X) can be quantified with the kit, irrespective of the library type, how it was constructed, or on which Illumina instrument it will be sequenced. The kit supports quantification of libraries across a range of GC contents and average fragment lengths up to 1 kb. In addition to NGS library quantification, the kit can also be used to detect library contamination in work spaces used during the preparation of Illumina libraries. The KAPA Library Quantification assay consists of repetitive pipetting steps, which can easily be automated. The use of an automated liquid handling system is highly recommended for high-throughput NGS pipelines. Please refer to the KAPA Library Quantification Technical Guide for more information. en

User profile

Select your user profile

KAPA Library Quantification Kit

Overview

Ordering information

Technical documents

Compatible products

Contact us