For Research Use Only. Not for use in diagnostic procedures. Others KAPA RNA EvoPrep Kit RUO KAPA RNA EvoPrep Kit PID00001115 featuring the KAPA EvoT4 DNA Ligase The KAPA RNA EvoPrep Kit is designed for both manual and automated NGS library construction from 0.25 – 100 ng of purified total, mRNA-enriched, or rRNA-depleted RNA. This workflow is compatible with both high- and low-quality (e.g., extracted from FFPE tissue) RNA. The protocol is applicable to a wide range of RNA-Seq applications, including: gene expression analysis; single nucleotide variation (SNV) discovery; splice junction and gene fusion identification; and characterization of both polyadenylated and non-polyadenylated RNAs, including noncoding and immature RNAs. This kit has not been tested with small RNAs < 100 bp in length. en The KAPA RNA EvoPrep Workflow provides:Improvements over the KAPA RNA HyperPrep Kit, due to the optimization of reagents and workflowConvenient, once-off preparation of 2nd strand synthesis and A-tailing master mixReady-to-use Ligation ReadyMixOne post-ligation bead cleanupReagents with improved shelf-life and stabilityShortened workflow time and minimal hands-on timeAn easy-to-use, streamlined, and automation-friendly workflow with minimal resource requirementsSingle vendor service when using the following accessory reagents:KAPA Unique Dual-Indexed AdaptersKAPA Universal Adapter with KAPA UDI Primer Mixes 1 - 384 en

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