For Research Use Only. Not for use in diagnostic procedures. Others KAPA Unique Dual-Indexed Adapter Kit RUO KAPA Unique Dual-Indexed Adapter Kit SEQ-KAPA-0223 08 861 919 702 8 861 919 702 08861919702 8861919702 08861919702 KAPA UDI Adapters Kit 15uM KAPA Unique Dual-Indexed Adapter Kit 07613336168967 Reagents, kits KK8727 with KAPA Adapter Dilution Buffer Not Available KAPA Unique Dual-Indexed Adapters are used to uniquely label sequencing libraries generated from individual biological samples. This allows for the pooling of libraries prior to target capture or cluster generation, to enable multiplexed sequencing; which simplifies sample preparation and reduces the cost of next-generation sequencing for a wide range of applications. Primary applications for the use of KAPA Unique Dual-Indexed Adapter Kit for Illumina platforms include: Human whole genome sequencing, particularly PCR-free workflows whole-exome or targeted sequencing, using hybridization capture systems (in combination with compatible blockers) RNA-seq ChIP-seq other direct sequencing applications, e.g., microbial whole-genome sequencing on compatible platforms NOTE: KAPA Unique Dual-Indexed Adapters are not methylated, and can therefore not be used for Methyl-Seq applications. Pooling/Multiplexing Guidelines NovaSeq X/Plus: please contact your local Roche Technical Support (sequencing.roche.com/support) for comprehensive pooling guidelines. All other Illumina sequencers: please see detail in IFU (method sheet) en KAPA Unique-Dual Indexed Adapters comprise a set of 96 full-length adapters used during ligation-based library construction for sequencing. Each KAPA Unique Dual-Indexed Adapter contains two, non-redundant, 8-nucleotide indexes (sequencing barcodes) for multiplexed sequencing applications. These adapters are designed for use with KAPA DNA and RNA library preparation kits. Each KAPA Unique Dual-Indexed Adapter has been manufactured by duplexing (hybridizing) two oligonucleotides. The backbones of these oligonucleotides are identical to those employed in Illumina TruSeq® adapters, however the sequences of the sample indexes included in KAPA Unique Dual-Indexed Adapters are exclusive to Roche, and differ from those employed by other suppliers. The 192 non-redundant indexes combined to create 96 Unique Dual-Indexed Adapters are listed in Table 2*. KAPA Unique Dual- Indexed Adapters are designed and formulated to ensure high library construction efficiency and low adapter- dimer formation, and mitigate the technical challenges associated with index misalignment (“index hopping”) on Illumina sequencers that employ patterned flow cells and exclusion amplification chemistry.1 KAPA Adapter Dilution Buffer [10mM Tris-HCl, (pH8.0– 8.5), 10mM NaCl, 1mM EDTA] is provided with the kit to ensure optimal performance when adapters require further dilution. *see method sheet for Table 2 1Illumina. Effects of Index Misassignment on Multiplexing and Downstream Analysis. 2017 en

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