The Regional Virus Laboratory (RVL), located at Belfast’s Royal Hospitals Complex, is the only UKAS-accredited provider of clinical virology testing services for hospitals, GPs and public health professionals in Northern Ireland. Serving a population of 1.8 million and around 1600 users, the RVL offers a broad range of conventional serology, antigen detection and molecular tests for the diagnosis of viral, bacterial and other infections. Since 2014, the RVL Molecular Section has used the Roche FLOW Solution for the molecular analysis of a wide range of infectious diseases. The laboratory offers a complex portfolio of around 40 laboratory developed tests (LDT) PCR assays, both qualitative and quantitative, that target viral and bacterial DNA or RNA—preparing their own master mixes and using universal PCR amplification conditions.