Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Cytoplasm Others Glypican-3 1G12 IVD Hepatocellular Carcinoma Glypican-3 (1G12) Mouse Monoclonal Antibody RTD000931 Mouse 1G12 05973864001 Glypican-3 (1G12) PAb, Cell Marque Glypican-3 (1G12) 00841683100997 Reagents, kits 50 tests Not Available true Glypican-3 (1G12) Mouse Monoclonal Primary Antibody ist für den Einsatz im Labor zum Nachweis des Proteins Glypican-3 in formalinfixiertem, paraffineingebettetem menschlichem Gewebe vorgesehen, das in qualitativen immunhistochemischen (IHC) Tests auf BenchMark IHC/ISH-Geräten gefärbt wurde. Dieses Produkt sollte von einem qualifizierten Pathologen in Verbindung mit einer histologischen Untersuchung, relevanten klinischen Informationen und ordnungsgemäßen Kontrollen interpretiert werden. Dieser Antikörper ist für den in-vitro-diagnostischen (IVD) Gebrauch bestimmt. de Glypican-3 (1G12) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual. en