For in vitro diagnostic use. Others ISH Protease 2 IVD ISH Protease 2 RTD001007 05273323001 ISH PROTEASE 2 ISH Protease 2 04015630971879 Reagents, kits 780-4148 200 tests Not Available true ISH Protease 2 is used with probes, accessory reagents and the BenchMark IHC/ISH instrument to achieve appropriate in situ hybridization (ISH) staining.Enzymatic digestion using ISH Protease 2 removes cross-linking of proteins to ensure that the probe of interest reaches its target DNA or RNA sequence.1 ISH Protease 2 is a medium activity enzyme formulation that cleaves proteins in the formalin-fixed, paraffinembedded (FFPE) tissue section, exposing the target nucleic acid sequence for hybridization by the probe. Digestion is followed by probe application and is used in combination with VENTANA detection kits. ISH Protease 2 may be used for target nucleic acid sequences requiring moderate digestion for optimal staining.1. Beesley JE. Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences. Boston: Birkhäuser, 2001. en ISH Protease 2 ist für den enzymatischen Aufschluss von Schnitten von formalinfixiertem, paraffineingebettetem Gewebe oder Präparaten mit ganzen Zellen auf einem BenchMark IHC/ISH Gerät vorgesehen. Bei der In-situ-Hybridisierung entfernt dieses Reagenz Protein, das die relevanten Zielnukleinsäuresequenzen umgibt. Dieses Reagenz ist für die Verwendung in der In-vitro-Diagnostik (IVD) bestimmt. de