For in vitro diagnostic use. Others VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail IVD VENTANA® Kappa and Lambda Dual ISH mRNA Probe Cocktail PID00000773 CE-IVD 800-6054 VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail 07613336160558 30 tests true VENTANA K L DISH mRNA PRB CKT-USExport 08507023001 Not Available Reagents, kits de VENTANA Kappa und Lambda Dual ISH mRNA Probe Cocktail ist für den qualitativen Nachweis von Kappa-mRNA und Lambda-mRNA in formalinfixiertem, paraffineingebettetem (FFPE) menschlichem Knochenmark und lymphatischem Gewebe, das mit einem BenchMark IHC/ISH-Gerät mittels chromogener In-situ- Hybridisierung (ISH) gefärbt und lichtmikroskopisch sichtbar gemacht wurde. VENTANA Kappa and Lambda Dual ISH mRNA Probe Cocktail ist als Hilfsmittel für die Identifizierung von B- Zell-Lymphomen und Plasmazell- Neoplasmen bestimmt.Die Ergebnisse des Assays müssen von einem qualifizierten Pathologen in Verbindung mit histologischen Untersuchungen, klinisch relevanten Informationen und geeigneten Kontrollen interpretiert werden.Dieses Produkt ist für die Verwendung in der In-vitro-Diagnostik (IVD) bestimmt. en VENTANA K/L Probe Cocktail is formulated for use with the VENTANA Magenta ISH DIG Detection Kit, the VENTANA Silver ISH BF Detection Kit, the ISH TSA Ancillary Kit, and accessory reagents on a BenchMark IHC/ISH instrument.The detection kits contain HRP-labeled anti-hapten primary antibody, hapten-labeled tyramide amplification reagent, and silver or magenta chromogen. During the ISH staining process, BF and DIG-labeled probes are hybridized to their respective target sequences in the tissue.DIG-labeled kappa probe is detected with VENTANA Magenta ISH DIG Detection Kit. This detection system uses HRP-labeled anti-DIG mouse monoclonal antibody and DIG- labeled tyramide amplification to visualize the target as a magenta signal through the covalent linkage of sulforhodamine B labeled tyramide to the tissue (see Figure 2).Figure 2. VENTANA Magenta ISH DIG Detection Kit BF-labeled lambda probe is detected with VENTANA Silver ISH BF Detection Kit. This detection system uses HRP-labeled anti-BF mouse monoclonal antibody and BF-labeled tyramide amplification to visualize the target as a black signal through the precipitation of silver (see Figure 3).Figure 3. VENTANA Silver ISH BF Detection Kit Refer to the method sheets of these detection reagents for additional information.The staining protocol consists of numerous steps in which reagents are incubated for pre- determined times at specific temperatures. At the end of each incubation step, the BenchMark instrument washes the sections to remove unbound material and applies a liquid coverslip to minimize evaporation of the aqueous reagents from the slide. Results are interpreted using a light microscope.For more detailed information on instrument operation, refer to the appropriate User Guide.