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MagNA Pure 96 Instrument
2. Control Unit (Desktop PC, 19'' monitor, keyboard, mouse, 2nd handheld barcode scanner)
3. Preinstalled MagNA Pure 96 operating software and purification protocols
4. Operator's Guide", "Name": "Content" }, { "Language": "fr", "Value": "L'instrument MagNA Pure 96 est conçu pour effectuer une extraction automatique des acides nucléiques à des fins de diagnostic in vitro. L'instrument MagNA Pure 96 est destiné à une utilisation conjointe avec les kits spécifiques MagNA Pure 96.
Le système MagNA Pure 96 est destiné à une utilisation avec la station de travail automatisée définie, un ordinateur (unité de contrôle) prenant en charge le logiciel, un protocole logiciel, un kit de préparation d'échantillon et des consommables. Il doit être utilisé par des professionnels uniquement.", "Name": "Intended Use" } ] } } ] }

Système MagNA Pure 96

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Image produit du système MagNA Pure 96
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Une purification sans effort en toute confiance grâce à l’automatisation
  • Instrument à haut débit pour la purification automatisée des acides nucléiques
  • Des performances éprouvées pour des rendements reproductibles et constants
  • Traitez jusqu’à 96 échantillons en moins d’une heure
  • Prise en charge d’une large gamme d’échantillons pour une utilisation dans diverses applications ultérieures
  • Flux de travail simplifié grâce à des plateaux de réactifs préremplis prêts à l’emploi et des offres de kits simplifiés
  • Traçabilité complète et connexion à votre système d’information de laboratoire
  • Réduction des erreurs de manipulation grâce à des processus de détection, de guidage et de surveillance
  • Prévention de la contamination grâce à la lampe UV intégrée et au collecteur de gouttes, ainsi qu’à la technologie CO-RE Tip

 

Kits de réactifs préremplis, à code-barres et prêts à l’emploi

 

Les kits MagNA Pure 96 sont des plateaux de réactifs préremplis et prêts à l’emploi qui peuvent être facilement chargés, garantissant ainsi une facilité d’utilisation et une sécurité optimales. Les kits et les consommables sont codés et lus par le scanner MagNA Pure intégré pour la vérification de l’inventaire, les erreurs de chargement et la documentation du processus.

 

Une interface utilisateur intuitive

 

Le logiciel MagNA Pure 96 comprend une interface utilisateur graphique intuitive, avec toutes les instructions pour faire fonctionner le système, comme le chargement des échantillons en début d’analyse. Le logiciel détecte les erreurs de chargement et fournit des informations à l’utilisateur pour lui permettre de prendre les mesures correctives appropriées.

 

Une nouvelle dimension de la productivité

 

Faites l’expérience d’une automatisation à haut débit associée à des performances élevées tout en augmentant la productivité de votre laboratoire pour répondre à vos besoins changeants. Augmentez l’efficacité de votre laboratoire grâce à une gestion transparente des données et augmentez votre capacité pour répondre aux divers besoins quotidiens de débit.

Statut d’enregistrement

DIV

Notices d’utilisation

Les notices d’utilisation sont disponibles sur le site Internet de Roche Diagnostics de votre pays.

Aperçu

Commander

Documents techniques

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Protocoles du système MagNA Pure 96

Les protocoles du système MagNA Pure 96 sont téléchargeables dans le tableau ci-dessous (remarque : non téléchargeables aux États-Unis).

Les applications sont très flexibles, en raison de la diversité des protocoles de purification logicielle utilisant des plateaux préremplis à code-barres avec des réactifs prêts à l’emploi.

Les kits MagNA Pure 96 sont conçus pour tirer profit de notre expertise de plus de dix ans dans la technologie des particules de verre magnétique. Nous avons simplifié le processus de purification en un très petit nombre de réactifs, consommables et étapes.

Les protocoles de purification préprogrammés optimisés garantissent une manipulation d’échantillons de routine et des rendements d’acides nucléiques reproductibles de haute qualité. Choisissez le protocole de purification approprié pour le matériau de votre échantillon et l’acide nucléique cible.

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Target Protocol name Input sample type and sample volumes
The latest version of MagNA Pure 96 protocols are available to download from the table below (not available in US)
Elution volume Run time*** (h:mm)
Whole blood Plasma (citrate) Plasma (EDTA) Serum Swabs BAL*
Sputum CSF Urine Stool Cultured cells Fresh-frozen tissue FFPET**
Bacterial, fungal and viral NA Pathogen Universal 200 1 200 μL 50, 100 μL 1:00
Viral NA Plasma SV 1 50, 100, 200 μL 1:00
Viral NA Plasma ext lys SV 1

Protocol optimized with external lysis reagent

350, 450 μL lysate from 100, 200 μL sample 1:00
Viral NA Universal SV 1 50, 100, 200 μL 50, 100, 200 μL 1:00
Pathogen Universal 500 2 500 μL 1:30
Pathogen Universal 1000 2 1000 μL 1:40
Viral NA Plasma LV 2 500 μL 1:10
Viral NA Plasma LV 1000 2 1000 μL 1:20
Viral NA Plasma ext lys LV 2

Protocol optimized with external lysis reagent

1000 μL lysate from 500 μL sample 1:10
Viral NA Universal LV 2 500 μL 500 μL 1:30
Viral NA Universal LV 1000 2 1000 μL 1000 μL 1:30
Genomic DNA DNA Blood SV 1

Recommended for elution of predominantly double-stranded DNA

50, 100 or 200 μL ≤ 2x106 cells/samples 50, 100 μL 1:00
DNA Blood ext lys SV 1

Protocol optimized with external lysis reagent

300, 350, or 450 μL lysate from
50, 100, 200 μL sample
0:50
DNA Cells SV 1 Up to 5x105 cultured cells in 200 μL PBS 100 μL 1:00
DNA Tissues SV 1 Up to 5 mg in 200 μL 100, 200 μL 1:20
DNA FFPE SV 1 200 μL lysate from 2x5 μL sections 50, 100 μL 1:00
DNA Blood LV 2 500 μL (≤ 1x107 cells/ml) 100, 200 μL 1:20
DNA Blood LV 1000 2 1000 μL (≤ 1x107 cells/ml) 1:30
DNA Blood ext lys LV 2

Protocol optimized with external lysis reagent

1000 μL lysate from 500 μL sample (≤ 1x107 cells/ml) 100, 200 μL 0:50
DNA Cells LV 1 Up to 1x106 cultured cells in 200 μL PBS 100, 200 μL 1:20
DNA Tissue LV 2 Up to 25 mg in 200 μL 200 μL 1:20
Cell-free NA cfNA ss 2000 2

Protocol optimized for single-stranded DNA

2000 μL 50, 100 μL 2:00
cfNA ss 4000 2

Protocol optimized for single-stranded DNA

4000 μL 2:20
cfNA ds 2000 2

Protocol optimized for single-stranded DNA

2000 μL 100, 200 μL 2:20
cfNA ds 4000 2

Protocol optimized for single-stranded DNA

4000 μL 2:50
Total RNA Cellular RNA LV 3 Up to 1x106 cultured cells in 200 μL PBS 50, 100, 200 μL 1:30
RNA Blood LV 400 3 900 μL stabilized whole blood from 400 μL whole blood 50, 100, 200 μL 1:30
RNA Blood LV 800 3 1800 μL stabilized whole blood from 800 μL whole blood 100, 200 μL 1:30
RNA PAXgene LV 3 PAXgene pellet in 400 μL PBS 100, 200 μL 1:30
RNA PAXgene Half Tube LV 3 Half PAXgene pellet in 200 μL PBS 50, 100 μL 1:30
RNA Tissue FF Standard LV 3 Up to 10 mg in 350 μL 50, 100, 200 μL 1:30
RNA Tissue FF High LV 3 Up to 25 mg in 700 μL 100, 200 μL 1:30
RNA Tissue FFPE LV 3 150 μL lysate from 2x5 μm sections 50, 100 μL 1:30
Liquid handling Sample Transfer
to the processing cartridge
50 µL to 1000 µL NA
Archive Plate Transfer
for archiving purposes
5 μL to 200 μL NA
PCR Setup with Mixing NA
PCR Setup without Mixing NA

1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001)
2 MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)
3 MagNA Pure 96 Cellular RNA Large Volume Kit (cat no. 05 467 535 001)

To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set (Cat. no. 07 794 398 001), MagNA Pure External Lysis Buffer (Cat. no. 06 374 913 001), MagNA Pure Bacterial Lysis Buffer (Cat. no. 06 374 921 001), MagNA Pure DNA Tissue Lysis Buffer (Cat. no. 06 640 702 001), MagNA Pure FFPET Buffer Set† (Cat. no. 08 447 144 001) and S.T.A.R. Buffer§ (Cat. no. 03 335 208 001). Please refer to the instructions for use manual for more details.

*BAL - Bronchoalveolar Lavage
**FFPET - formalin-fixed paraffin-embedded tissue
***These times are approximate and should only be used as a guidance.
For general laboratory use.
§For life science research only. Not for use in diagnostic procedures.

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