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(Product)", "Value": "Upon receipt and when not in use, store at 2-8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "Le bcl-6 (GI191E/A8) Mouse Monoclonal Antibody est destiné à un usage en laboratoire pour détecter la protéine bcl6 dans les tissus humains fixés au formol et enrobés à la paraffine, colorés en immunohistochimie (IHC) qualitative sur les instruments BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "BCL6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein.¹ In normal tissue, anti-BCL6 antibody shows strong nuclear staining in a subset of B-lymphocytes, mostly located in germinal centers (GC).² BCL6 expression is usually present in follicular lymphomas,³ diffuse large B-cell lymphomas, and Burkitt lymphomas.⁴

1. Falini, B et al. “Bcl-6 protein expression in normal and neoplastic lymphoid tissues.” Annals of oncology : official journal of the European Society for Medical Oncology vol. 8 Suppl 2 (1997): 101-4.
2. Aukema, Sietse M et al. “Double-hit B-cell lymphomas.” Blood vol. 117,8 (2011): 2319-31.
3. Dogan, A et al. “CD10 and BCL-6 expression in paraffin sections of normal lymphoid tissue and B-cell lymphomas.” The American journal of surgical pathology vol. 24,6 (2000): 846- 52.
4. Carbone, Antonino et al. “B-cell lymphomas with features intermediate between distinct pathologic entities. From pathogenesis to pathology.” Human pathology vol. 41,5 (2010): 621-31.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Principle", "Value": "bcl-6 (GI191E/A8) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "Le bcl-6 (GI191E/A8) Mouse Monoclonal Antibody est destiné à un usage en laboratoire pour détecter la protéine bcl6 dans les tissus humains fixés au formol et enrobés à la paraffine, colorés en immunohistochimie (IHC) qualitative sur les instruments BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }