For in vitro diagnostic use. Others ISH Protease 2 IVD ISH Protease 2 RTD001007 05273323001 ISH PROTEASE 2 ISH Protease 2 04015630971879 Reagents, kits 780-4148 200 tests Not Available true ISH Protease 2 is used with probes, accessory reagents and the BenchMark IHC/ISH instrument to achieve appropriate in situ hybridization (ISH) staining.Enzymatic digestion using ISH Protease 2 removes cross-linking of proteins to ensure that the probe of interest reaches its target DNA or RNA sequence.1 ISH Protease 2 is a medium activity enzyme formulation that cleaves proteins in the formalin-fixed, paraffinembedded (FFPE) tissue section, exposing the target nucleic acid sequence for hybridization by the probe. Digestion is followed by probe application and is used in combination with VENTANA detection kits. ISH Protease 2 may be used for target nucleic acid sequences requiring moderate digestion for optimal staining.1. Beesley JE. Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences. Boston: Birkhäuser, 2001. en L’ISH Protease 2 est destinée à être utilisée pour la digestion enzymatique sur un appareil BenchMark IHC/ISH des coupes de tissus fixés au formol et inclus en paraffine ou des préparations de cellules entières. Ce réactif élimine les protéines qui entourent les séquences d’acide nucléique cibles lors des applications d’hybridation in situ. Ce réactif est conçu pour une utilisation en diagnostic in vitro (IVD). fr