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The antibody is diluted in Tris-HCI with carrier protein, and 0.10% ProClin 300, a preservative.
Specific antibody concentration is approximately 6.7 μ g/mL.
CONFIRM anti-Kappa antibody is a rabbit polyclonal antibody produced as rabbit serum.
Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.",
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CONFIRM anti-Kappa antibody may be used to detect Kappa light chain protein expression in plasma cells by immunohistochemistry (IHC). Determination of the ratio of Kappa to Lambda light chain expression in this cell population may be used to aid in the differentiation between a reactive process and a plasma cell neoplasm. This antibody may be used as part of a panel of IHC studies.The staining pattern is cytoplasmic and/or membraneous.
1. Melchers F. Checkpoints That Control B Cell Development. J Clin Invest. 2015;125(6):2203-2210.
2. Lucas JS, Murre C, Feeney AJ, et al. The Structure and Regulation of the Immunoglobulin Loci. In: Alt FW, Honjo T, Radbruch A, Reth M, eds. Molecular Biology of B Cells.2015:1-11.
3. Pieper K, Grimbacher B, Eibel H. B-Cell Biology and Development. J Allergy Clin Immunol. 2013;131(4):959-971.
4. O'Malley DP, Fedoriw Y, Grimm KE, et al. Immunohistology of Lymph Node and Lymph Node Neoplasms, 5th Edition. In: Dabbs DJ, ed. Diagnostic Immunohistochemistry. Elsevier2019:160-202.
5. Higgins RA, Blankenship JE, Kinney MC. Application of Immunohistochemistry in the Diagnosis of Non-Hodgkin and Hodgkin Lymphoma. Arch Pathol Lab Med. 2008;132(3):441-461.
6. Garcia CF, Swerdlow SH. Best Practices in Contemporary Diagnostic Immunohistochemistry Panel Approach to Hematolymphoid Proliferations. Arch Pathol Lab Med. 2009;133(5):756-765.",
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