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[ { "ProductSpecVariant": { "Chapters": [ { "Name": "Storage Conditions (Product)", "Value": "Store at 2-8° C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Content", "Value": "CONFIRM anti-Lambda antibody contains sufficient reagent for 50 tests.

One 5 mL dispenser of CONFIRM anti-Lambda antibody contains approximately 22 μg of a rabbit polyclonal antibody.

The antibody is diluted in Tris-HCI with carrier protein, and 0.10% ProClin 300, a preservative.

Specific antibody concentration is approximately 4.4 μg/mL.

CONFIRM anti-Lambda antibody is a rabbit polyclonal antibody produced as rabbit serum.
Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Intended Use", "Value": "Le CONFIRM anti-Lambda Rabbit Polyclonal Primary Antibody est destiné à être utilisé en laboratoire pour la détection immunohistochimique qualitative par microscopie optique des chaînes légères Lambda dans des coupes de tissus fixés au formol et inclus en paraffine colorées sur un appareil BenchMark IHC/ISH.

Ce produit doit être interprété par un anatomopathologiste qualifié, en complément d’examens histologiques, d’informations cliniques pertinentes et de contrôles adaptés.

Cet anticorps est conçu pour une utilisation en diagnostic in vitro (IVD).

", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Principle", "Value": "CONFIRM anti-Lambda antibody binds to human lambda light chain protein in formalin-fixed, paraffin-embedded (FFPE) tissue sections. This antibody can be visualized using ultraView Universal DAB Detection Kit (Cat. No. 760-500 / 05269806001) or OptiView DAB IHC Detection Kit (Cat. No. 760-700 / 06396500001). Refer to the respective method sheets for further information.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Background Information", "Value": "CONFIRM anti-Lambda Rabbit Polyclonal Primary Antibody (CONFIRM anti-Lambda antibody) detects Lambda light chains. Lambda light chains are polypeptide chains, which in combination with heavy chains, form immunoglobulin molecules.^1,2,3There are two classes of light chains found in immunoglobulins: Kappa light chains and Lambda light chains.^2,3Light chain production by lymphoid cells is genetically restricted such that the immunoglobulin molecules produced by an individual cell will only contain a single light chain class (either Kappa or Lambda) but not both.^2,3 Plasma cell neoplasms often exhibit light chain restriction, in which the normal ratio of Kappa to Lambda immunoglobulin light chain expressing plasma cells is skewed.^3-6 Detection of clonal light chain restriction based on the Kappa to Lambda ratio is often used to differentiate monoclonal proliferations, which are often malignant, from polyclonal proliferations that arise during normal or reactive immune response.^3-6

CONFIRM anti-Lambda antibody may be used to detect Lambda light chain protein expression in plasma cells by immunohistochemistry (IHC). Determination of the ratio of Kappa to Lambda light chain expression in this cell population may be used to aid in the differentiation between a reactive process and a plasma cell neoplasm. This antibody may be used as part of a panel of IHC studies. The staining pattern is cytoplasmic and/or membranous.

1. Melchers F. Checkpoints That Control B Cell Development. J Clin Invest. 2015;125(6):2203-2210.
2. Lucas JS, Murre C, Feeney AJ, et al. The Structure and Regulation of the Immunoglobulin Loci. In: Alt FW, Honjo T, Radbruch A, Reth M, eds. Molecular Biology of B Cells.2015:1-11.
3. Pieper K, Grimbacher B, Eibel H. B-Cell Biology and Development. J Allergy Clin Immunol. 2013;131(4):959-971.
4. O'Malley DP, Fedoriw Y, Grimm KE, et al. Immunohistology of Lymph Node and Lymph Node Neoplasms, 5th Edition. In: Dabbs DJ, ed. Diagnostic Immunohistochemistry. Elsevier2019:160-202.
5. Higgins RA, Blankenship JE, Kinney MC. Application of Immunohistochemistry in the Diagnosis of Non-Hodgkin and Hodgkin Lymphoma. Arch Pathol Lab Med. 2008;132(3):441-461.
6. Garcia CF, Swerdlow SH. Best Practices in Contemporary Diagnostic Immunohistochemistry Panel Approach to Hematolymphoid Proliferations. Arch Pathol Lab Med. 2009;133(5):756-765.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Product Purpose", "Value": "Le CONFIRM anti-Lambda Rabbit Polyclonal Primary Antibody est destiné à être utilisé en laboratoire pour la détection immunohistochimique qualitative par microscopie optique des chaînes légères Lambda dans des coupes de tissus fixés au formol et inclus en paraffine colorées sur un appareil BenchMark IHC/ISH.

Ce produit doit être interprété par un anatomopathologiste qualifié, en complément d’examens histologiques, d’informations cliniques pertinentes et de contrôles adaptés.

Cet anticorps est conçu pour une utilisation en diagnostic in vitro (IVD).

", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }

CONFIRM anti-Lambda Rabbit Polyclonal Primary Antibody

IVD

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