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receipt and when not in use, store at 2-8°C. Do not freeze.", "Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "Le PGP 9.5 Rabbit Polyclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la enzyme PGP9.5 (UCH-L1) dans les tissus fixés au formol et enrobés à la paraffine, colorés sur les instruments VENTANA BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Intended Use" }, { "Name": "Background Information", "Value": "Protein gene product 9.5 (PGP 9.5), also known as ubiquitin carboxyl-terminal hydrolase-1 (UCHL-1), is a 27 kDa protein originally isolated from whole brain extracts.¹ Although PGP 9.5 expression in normal tissues was originally felt to be strictly confined to neurons and neuroendocrine cells,² it has been subsequently documented in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts.¹ A plethora of mesenchymal neoplasms have demonstrated PGP 9.5 expression.^1,3,4

1. Campbell LK, et al. Protein gene product 9.5 (PGP 9.5) is not a specific marker of neural and nerve sheath tumors: an immunohistochemical study of 95 mesenchymal neoplasms. Mod Pathol. 2003; 16:963-9.
2. Bassotti G, et al. Interstitial cells of Cajal, enteric nerves, and glial cells in colonic diverticular disease. J Clin Pathol. 2005; 58:973-7.
3. Mahalingam M, et al. Expression of PGP 9.5 in granular cell nerve sheath tumors: and immunohistochemical study of six cases. J Cutan Pathol. 2001; 28:282-6.
4. Mahalingam M, et al. Multiple cellular neurothekeomas—a case report and review on the role of immunohistochemistry as a histo-logic adjunct. J Cutan Pathol. 2006; 33:51-6.", "Language": "en", "Country": "XG", "Code": "Background Information" }, { "Name": "Content", "Value": "One dispenser of this antibody contains sufficient prediluted reagent for 50 tests.", "Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Principle", "Value": "PGP 9.5 Rabbit Polyclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.

This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specifi c temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual.", "Language": "en", "Country": "XG", "Code": "Principle" }, { "Name": "Product Purpose", "Value": "Le PGP 9.5 Rabbit Polyclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la enzyme PGP9.5 (UCH-L1) dans les tissus fixés au formol et enrobés à la paraffine, colorés sur les instruments VENTANA BenchMark IHC/ISH. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD).", "Language": "fr", "Country": "XG", "Code": "Product Purpose" } ] } } ] }