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{
"Name": "Background Information",
"Value": "The VENTANA PTEN (SP218) Rabbit Monoclonal Primary Antibody (VENTANA PTEN (SP218) antibody) is directed against thedual specificity protein phosphatase encoded by the tumor suppressor gene phosphatase and tensin homolog (PTEN) at chromosome 10q23.3.1,2 PTEN mediated dephosphorylation is important because it results in inhibition of the Akt signaling pathway, which plays an important role in controlling cell survival and cell cycle progression.3,4 The PTEN protein is localized in the cytoplasm and nucleus, and is an essential enzyme expressed in severalnon-neoplastic tissues.1,5 Reduced or absent PTEN protein expression has been identified by immunohistochemical analyses in several cancers, including prostate, breast, thyroid, endometrial, gastric, non-small cell lung, and pancreatic.6-12
PTEN is one of the most commonly lost tumor suppressors in prostate cancer.13,14 The loss of PTEN protein expression in prostate cancer is frequently associated with advanced disease.15,16 The assessment of PTEN protein expression by immunohistochemistry (IHC) may be used to aid in the characterization of an advanced phenotype in prostate cancer.13-16 Although PTEN protein loss is correlated with clinicopathological features of advanced prostate cancer, it does not provide independent prognostic information.13,15
Results of a PTEN IHC assay are intended to provide supplemental information regarding the tumor phenotype and are to be interpreted in the context of established prognostic indicators.
1. Li J, Yen C, Liaw D, Podsypanina K, Bose S, Wang SI, Puc J, Miliaresis C, Rodgers L, McCombie r, Bigner SH, Giovanella BC, Ittmann M, Tycko B, Hibshoosh H, Wigler MH, Parson R. PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer. Science. 1997;275:1943-1947.
2. SteckPA, Pershouse MA, Jasser SA, Yung WKA, Lin H, Ligon AH, Langford LA, Baumgard ML, Hattier T, Davis T, Frye C, Hu R, Swedlund B, Teng DHF, Tavtigian SV. Identification of a candidate tumour suppressor gene, MMAC1, at chromosome 10q23.3 that is mutated in multiple advanced cancers. Nat Genet 1997;15:356-362.
3. Wu X, Senechal K, Neshat MS, Whang YE, Sawyers CL. The PTEN/MMAC1 tumor suppressor phosphatase functions as a negative regulator of the phosphoinositide 3-kinase/Akt pathway. Proc Natl Acad Sci. 1998;95:15587-15591.
4. Stambolic V, Suzuki A, de la Pompa JL, Brothers GM, Mirstos C, Sasaki T, Ruland J, Penninger JM, Siderovski DP, Mak TW. Negative regulation of PKB/Akt- dependent cell survival by the tumor suppressor PTEN. Cell. 1998;95:29-39.
5. Tamguney T, Stokoe D. New Insights into PTEN. J Cell Sci. 2007;120(Pt 23):4071- 4079.
6. Whang YE, Wu X, Suzuki H, Reiter RE, Tran C, Vassella RL, Said JW, Isaacs WB, Sawyers CL. Inactivation of the tumor suppressor PTEN/MMAC1 in advanced human prostate cancer through loss of expression. Proc Natl Acad Sci. 1998;95:5246-5250.
7. Perren A, Weng L-P, Boag AH, Ziebold U, Thakore K, Dahia PLM, Komminoth P, Lees JA, Mulligan LM, Mutter GL, Eng C. Immunohistochemical evidence of loss of PTEN expression in primary ductal adenocarcinomas of the breast. Am J Pathol. 1999;155:1253-1260.
8. Gimm O, Perren A, Weng L-P, Marsh DJ, Yeh JJ, Ziebold U, Gil E, Hinze R, Delbridge L, Lees JA, Mutter GL, Robinson BG, Komminoth P, Dralle H, Eng C. Differential nuclear and cytoplasmic expression of PTEN in normal thyroid tissue, and benign and malignant epithelial thyroid tumors. Am J Pathol. 2000;156:1693- 1700.
9. MutterGL, Lin M-C, Fitzgerald JT, Kum JB, Baak JPA, Lees JA, Weng L-P, Eng C. Altered PTEN expression as a diagnostic marker for the earlest endometrial precancers. J Natl Cancer Inst. 2000;92:924 -931.
10. Fei G, Ebert MPA, Mawrin C, Leodolter A, Schmidt N, Dietzmann K, Malfertheiner P. Reduced PTEN expression in gastric cancer and in the gastric mucosa of gastric cancer relatives. Eur J Gastoenterol Hepatol 2002;14:297-303.
11. Soria J-C, Lee H-Y, Lee JI, Wang L, Issa J-P, Kemp BL, Liu DD, Kurie JM, Mao L, Khuri FR. Lack of PTEN expression in non-small cell lung cancer could be related to promoter methylation. Clin Cancer Res. 2002;8:1178-1184.
12. Asano T, Yao Y, Zhu J, Li D, Abbruzzese JL, Reddy SAG. The Pi 3-kinase/Akt signaling pathway is activated due to aberrant PTEN expression and targets transcription factors NF-κB and c -Myc in pancreatic cancer cells. Oncogene. 2004;23:8571-8580.
13. Jamaspishvili T, Berman DM, Ross AE, et al. Clinical Implications of PTEN Loss in Prostate Cancer. Nat Rev Urol. 2018;15(4):222-234.
14. Shah RB, Shore KT, Yoon J, et al. PTEN Loss in Prostatic Adenocarcinoma Correlates with Specific Adverse Histologic Features (Intraductal Carcinoma, Cribriform Gleason Pattern 4 and Stromogenic Carcinoma). Prostate. 2019;79(11):1267-1273.
15. Carneiro A, Barbosa ARG, Takemura LS, et al. The Role of Immunohistochemical Analysis as a Tool for the Diagnosis, Prognostic Evaluation and Treatment of Prostate Cancer: A Systematic Review of the Literature. Front Oncol. 2018;8:377.
16. McMenamin ME, Soung P, Perera S, et al. Loss of PTEN Expression in Paraffin- Embedded Primary Prostate Cancer Correlates with High Gleason Score and Advanced Stage. Cancer Res. 1999;59(17):4291-4296.",
"Language": "en",
"Country": "XG",
"Code": "Background Information"
},
{
"Name": "Content",
"Value": "VENTANA PTEN (SP218) antibody contains sufficient reagent for 50 tests.
One 5 mL dispenser of VENTANA PTEN (SP218) antibody contains approximately 15 μg of a rabbit monoclonal antibody.
The antibody is diluted in Tris buffered saline, EDTA, Brij-35 with carrier protein and sodium azide, a preservative.
Specific antibody concentration is approximately 3 μg/mL. There is no known non-specific antibody reactivity observed in this product.
VENTANA PTEN (SP218) antibody is a Protein A purified recombinant rabbit monoclonal antibody.
Refer to the appropriate VENTANA detection kit method sheet for detailed descriptions of: Principle of the Procedure, Material and Methods, Specimen Collection and Preparation for Analysis, Quality Control Procedures, Troubleshooting, Interpretation of Results, and Limitations.",
"Language": "en",
"Country": "XG",
"Code": "Content"
},
{
"Name": "Intended Use",
"Value": "Le VENTANA PTEN (SP218) Rabbit Monoclonal Primary Antibody est destiné à être utilisé en laboratoire pour la détection immunohistochimique qualitative par microscopie optique de la protéine PTEN (phosphatase and tensin homolog) dans des coupes de tissus fixés au formol et inclus en paraffine colorées sur un appareil BenchMark IHC/ISH.
Ce produit doit être interprété par un anatomopathologiste qualifié, en complément d’examens histologiques, d’informations cliniques pertinentes et de contrôles adaptés.
Cet anticorps est conçu pour une utilisation en diagnostic in vitro (IVD).",
"Language": "fr",
"Country": "XG",
"Code": "Intended Use"
},
{
"Name": "Principle",
"Value": "VENTANA PTEN (SP218) antibody binds to PTEN protein in sections of formalin-fixed, paraffin-embedded (FFPE) samples. The specific antibody can be localized using a haptenated secondary antibody followed by a multimer anti-hapten-HRP conjugate (OptiView DAB IHC Detection Kit, Cat. No.760-700 / 06396500001). The specific antibody-enzyme complex is then visualized with a precipitating enzyme reaction product. Refer to the OptiView DAB IHC Detection Kit method sheet for further information.",
"Language": "en",
"Country": "XG",
"Code": "Principle"
},
{
"Name": "Product Purpose",
"Value": "Le VENTANA PTEN (SP218) Rabbit Monoclonal Primary Antibody est destiné à être utilisé en laboratoire pour la détection immunohistochimique qualitative par microscopie optique de la protéine PTEN (phosphatase and tensin homolog) dans des coupes de tissus fixés au formol et inclus en paraffine colorées sur un appareil BenchMark IHC/ISH.
Ce produit doit être interprété par un anatomopathologiste qualifié, en complément d’examens histologiques, d’informations cliniques pertinentes et de contrôles adaptés.
Cet anticorps est conçu pour une utilisation en diagnostic in vitro (IVD).",
"Language": "fr",
"Country": "XG",
"Code": "Product Purpose"
}
]
}
}
]
}
