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"ProductSpec": [
{
"ProductSpecVariant": {
"Chapters": [
{
"Name": "Storage Conditions (Product)",
"Value": "Upon receipt and when not in use, store at 2-8°C. Do not freeze.",
"Language": "en",
"Country": "XG",
"Code": "Storage Conditions (Product)"
},
{
"Name": "Intended Use",
"Value": "Le CONFIRM anti-S100 (Polyclonal) Primary Antibody est un anticorps polyclonal de lapin destiné à être utilisé en laboratoire pour la détection immunohistochimique qualitative par microscopie optique de la protéine S100 dans des coupes de tissus fixés au formol et inclus en paraffine colorées sur un appareil BenchMark IHC/ISH. Ce produit doit être interprété par un anatomopathologiste qualifié, en complément d’examens histologiques, d’informations cliniques pertinentes et de contrôles adaptés. Cet anticorps est conçu pour une utilisation en diagnostic in vitro (DIV).",
"Language": "fr",
"Country": "XG",
"Code": "Intended Use"
},
{
"Name": "Content",
"Value": "CONFIRM anti-S100 (Polyclonal) antibody contains sufficient reagent for 50 tests.
One 5 mL dispenser of CONFIRM anti-S100 (Polyclonal) antibody contains approximately 50 μg of a rabbit polyclonal antibody.
The antibody is diluted in a phosphate buffered saline containing carrier protein and 0.05% ProClin 300 as a preservative.
Specific antibody concentration is approximately 10 μg/mL.
There is no known non-specific antibody reactivity observed in this product.",
"Language": "en",
"Country": "XG",
"Code": "Content"
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{
"Name": "Background Information",
"Value": "The S100 protein family consists of at least 25 low molecular weight (9–13 kDa) calcium- binding EF-hand proteins.1 The majority of these proteins are homo- and heterodimers in which the monomers are non-covalently bound.1 Members of the S100 family are expressed in a wide variety of cell types and are involved in regulating diverse intracellular processes such as contraction, motility, cell growth, cell cycle regulation, regulation of transcription factors, and protein phosphorylation.1,2 Select S100 proteins are also secreted and/or released by cellular damage and have extracellular functions.1,2
S100 is expressed in several cell types including melanocytes, astrocytes, Langerhans cells, cells of cartilaginous and adipose tissue, glial and neural cells, Schwann cells, and myoepithelial cells.1,3 Neoplasms derived from these cell types also express S100, such as melanomas, selected histiocytic proliferations, schwannomas, various carcinomas (e.g., salivary gland carcinomas, sweat gland carcinomas), gliomas, peripheral nerve sheath tumors (PNST).1,3
Detection of S100 protein by immunohistochemistry (IHC) with CONFIRM anti-S100 (Polyclonal) Primary Antibody (CONFIRM anti-S100 (Polyclonal) antibody) may be used as a melanocyte marker to aid in the differential diagnosis of melanocytic versus non- melanocytic tumors. It may be used as part of a panel of IHC studies. The staining pattern is cytoplasmic and nuclear.
1. Halawi A, Abbas O, Mahalingam M. S100 proteins and the skin: a review. J Eur Acad Dermatol Venereol. 2014;28(4):405-414.
2. Donato R, Cannon BR, Sorci G, et al. Functions of S100 proteins. Curr Mol Med. 2013;13(1):24-57.
3. Ordonez NG. Value of melanocytic-associated immunohistochemical markers in the diagnosis of malignant melanoma: a review and update. Human Pathology. 2014;45(2):191-205.",
"Language": "en",
"Country": "XG",
"Code": "Background Information"
},
{
"Name": "Principle",
"Value": "CONFIRM anti-S100(Polyclonal) antibody is a rabbit polyclonal antibody produced against purified bovine brain S100 protein. CONFIRM anti-S100 (Polyclonal) antibody binds to the S100 protein in formalin-fixed, paraffin-embedded tissue (FFPE) sections and exhibits a nuclear and cytoplasmic staining pattern. This antibody can be visualized using OptiView DAB IHC Detection Kit (Cat. No. 760-700 / 06396500001), ultra View Universal DAB Detection Kit (Cat. No. 760-500 / 05269806001), or ultraView Universal Alkaline Phosphatase Red Detection Kit (Cat. No. 760-501 / 05269814001). Refer to the respective method sheet for further information.",
"Language": "en",
"Country": "XG",
"Code": "Principle"
},
{
"Name": "Product Purpose",
"Value": "Le CONFIRM anti-S100 (Polyclonal) Primary Antibody est un anticorps polyclonal de lapin destiné à être utilisé en laboratoire pour la détection immunohistochimique qualitative par microscopie optique de la protéine S100 dans des coupes de tissus fixés au formol et inclus en paraffine colorées sur un appareil BenchMark IHC/ISH. Ce produit doit être interprété par un anatomopathologiste qualifié, en complément d’examens histologiques, d’informations cliniques pertinentes et de contrôles adaptés. Cet anticorps est conçu pour une utilisation en diagnostic in vitro (DIV).",
"Language": "fr",
"Country": "XG",
"Code": "Product Purpose"
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]
}
