Cell Marque - Instructions for UseCell Marque - Safety Data Sheet For in vitro diagnostic use. Nucleus Others Wilms Tumor Protein-1 6F-H2 IVD Kidney Testis Malignant Mesothelioma WT1 (6F-H2) Mouse Monoclonal Antibody RTD001244 Mouse 6F-H2 05435706001 WT1 (6F-H2) PAb, Cell Marque WT1 (6F-H2) 00841683100904 Reagents, kits 50 tests Not Available true Le WT1 (6F-H2) Mouse Monoclonal Primary Antibody est destiné à un usage en laboratoire pour détecter la protéine WT1 dans les tissus humains fixés au formol et enrobés à la paraffine colorés dans le cadre de tests d’immunohistochimie (IHC) qualitative sur les instruments d’IHC/ISH BenchMark. Ce produit doit être interprété par un pathologiste compétent en parallèle avec un examen histologique, les informations cliniques pertinentes et les contrôles appropriés. Cet anticorps est destiné à être utilisé aux fins de diagnostic in vitro (IVD). fr WT1 (6F-H2) Mouse Monoclonal Antibody (this antibody) may be used as the primary antibody for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. In general, immunohistochemical staining allows the visualization of antigens via the sequential application of a specific antibody (primary antibody) to the antigen, a secondary antibody (link antibody) to the primary antibody, an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and a coverslip applied. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.This antibody is optimally diluted to be compatible with VENTANA detection kits and BenchMark IHC/ISH instruments. Refer to the Tables in the Instructions for Use section for recommended staining protocols. Each step in the staining protocol includes incubation for a precise time at a specific temperature. At the end of each incubation step, the sections are rinsed by the BenchMark IHC/ISH instruments to stop the reaction and remove unbound material that would hinder the desired reaction in subsequent steps. To minimize evaporation of the aqueous reagents from the specimen-containing slide, a coverslip solution is applied in the slide stainer. For more detailed information on instrument operation, refer to the appropriate BenchMark IHC/ISH instruments Operator’s Manual. en